CD11c Recombinant Rabbit Monoclonal Antibody [PSH07-42] (HA722830)
Catalog# HA722830
Rabbit Monoclonal to CD11c
Tested for
-
WB
-
IHC-P
-
IHC-Fr
-
IF-Tissue
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CD11c on different lysates with Rabbit anti-CD11c antibody (HA722830) at 1/1,000 dilution.
Lane 1: RAW264.7 cell lysate (20 µg/Lane)
Lane 2:NIH/3T3 cell lysate (negative) (20 µg/Lane)
Lane 3: C2C12 cell lysate (negative) (20 µg/Lane)
Lane 4: Mouse spleen tissue lysate (30 µg/Lane)
Lane 5: Mouse colon tissue lysate (30 µg/Lane)
Predicted band size: 129 kDa
Observed band size: 140 kDa
Exposure time: Lane1-3: 3 minutes ; Lane 4-5: 25 seconds；ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722830) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: RAW264.7 cell lysate (20 µg/Lane)
Lane 2:NIH/3T3 cell lysate (negative) (20 µg/Lane)
Lane 3: C2C12 cell lysate (negative) (20 µg/Lane)
Lane 4: Mouse spleen tissue lysate (30 µg/Lane)
Lane 5: Mouse colon tissue lysate (30 µg/Lane)
Predicted band size: 129 kDa
Observed band size: 140 kDa
Exposure time: Lane1-3: 3 minutes ; Lane 4-5: 25 seconds；ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722830) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.