Exosome Essentials Antibody Sampler Kit (HAK21125)
Catalog# HAK21125
Reacts with
-
Human
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (HA721533) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate (negative)
Lane 3: MCF7 cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 20 kDa
Exposure time: Lane 1-4 (left): 53 seconds; Lane 1-4 (right): 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721533) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate (negative)
Lane 3: MCF7 cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 20 kDa
Exposure time: Lane 1-4 (left): 53 seconds; Lane 1-4 (right): 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721533) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.