CD63 Recombinant Rabbit Monoclonal Antibody [PSH06-64] (HA722731)
Catalog# HA722731
Rabbit Monoclonal to CD63
Tested for
-
WB
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IF-Cell
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FC
-
IP
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IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CD63 on different lysates with Rabbit anti-CD63 antibody (HA722731) at 1/1,000 dilution.
Lane 1: SK-MEL-28 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lane 3: U-87 MG cell lysate
Lane 4: HUVEC cell lysate
Lane 5: K-562 cell lysate
Lane 6: HEK-293 cell lysate
Lane 7: HL-60 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 30-65 kDa
Exposure time: Lane 1: 4 seconds; Lane 2-7: 18 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722731) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SK-MEL-28 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lane 3: U-87 MG cell lysate
Lane 4: HUVEC cell lysate
Lane 5: K-562 cell lysate
Lane 6: HEK-293 cell lysate
Lane 7: HL-60 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 30-65 kDa
Exposure time: Lane 1: 4 seconds; Lane 2-7: 18 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722731) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.