Coronavirus Host Cell Attachment and Entry Antibody Sampler Kit (HAK21097)
Catalog# HAK21097
Reacts with
-
Human
Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: 293T cell lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat kidney tissue lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 92 kDa
Observed band size: 100 kDa
Exposure time: 2 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: 293T cell lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat kidney tissue lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 92 kDa
Observed band size: 100 kDa
Exposure time: 2 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.