Polycomb Group Antibody Sampler Kit (HAK21086)
Catalog# HAK21086
Reacts with
-
Human
Western blot analysis of KMT6/EZH2 on different lysates with Rabbit anti-KMT6/EZH2 antibody (HA722095) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: 293T cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HeLa cell lysate
Lane 5: COS-1 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722095) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HEK-293 cell lysate
Lane 2: 293T cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HeLa cell lysate
Lane 5: COS-1 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722095) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.