RING1 Recombinant Rabbit Monoclonal Antibody [JE34-66]
Catalog# HA721461
RING1 Recombinant Rabbit Monoclonal Antibody [JE34-66]
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
RING1 Recombinant Rabbit Monoclonal Antibody [JE34-66]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human RING1 aa 150-250.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 42 kDa
Positive Control
THP-1 cell lysate, Jurkat cell lysate, 22RV1 cell lysate, human prostate tissue.
Conjugation
unconjugated
Clone Number
JE34-66
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:1,000
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IHC-P
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1:200
Target
Function
E3 ubiquitin-protein ligase RING1 is an enzyme that in humans is encoded by the RING1 gene. This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4.
Background References
1. Zhu K et al. Ring1 promotes the transformation of hepatic progenitor cells into cancer stem cells through the Wnt/β-catenin signaling pathway. J Cell Biochem. 2020 Aug
2. Zou JP et al. The E3 ubiquitin ligase RING1 interacts with COP9 Signalosome Subunit 4 to positively regulate resistance to root-knot nematodes in Solanum lycopersicum L. Plant Sci. 2022 Sep
Subcellular Location
Nucleus, Nucleus speckle.
UNIPROT
Synonyms
Ring1A antibody
E3 ubiquitin-protein ligase RING1 antibody
Polycomb complex protein RING1 antibody
Really interesting new gene 1 protein antibody
RING finger protein 1 antibody
Ring1 antibody
RING1_HUMAN antibody
RING1A antibody
Rnf1 antibody
Transcription repressor Ring1A antibody
Images
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Western blot analysis of RING1 on different lysates with Rabbit anti-RING1 antibody (HA721461) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: 22RV1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 50 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721461) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-RING1 antibody (HA721461) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721461) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"