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RING2 Recombinant Rabbit Monoclonal Antibody [JB38-41]

Usd: 385

Specification

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Catalog# ET7107-07

RING2 Recombinant Rabbit Monoclonal Antibody [JB38-41]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

Conjugation

  • unconjugated

Overview

Product Name

RING2 Recombinant Rabbit Monoclonal Antibody [JB38-41]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human RING2 aa 83-256 .

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 38 kDa

Positive Control

HeLa cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, human breast carcinoma tissue, mouse liver tissue, rat liver tissue.

Conjugation

unconjugated

Clone Number

JB38-41

RRID

AB_3070724

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:200

Target

Function

E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (Probable).

Background References

1. Lee S-J et al. E3 ligase activity of RING finger proteins that interact with Hip-2, a human ubiquitin-conjugating enzyme. FEBS Lett 503:61-64 (2001).

2. Wang H et al. Role of histone H2A ubiquitination in Polycomb silencing. Nature 431:873-878 (2004) .

Post-translational Modification

Polyubiquitinated in the presence of UBE2D3 (in vitro).; Monoubiquitinated, by auto-ubiquitination.

Subcellular Location

Chromosome. Nucleus.

UNIPROT

SwissProt: Q99496 Human

SwissProt: Q9CQJ4 Mouse

SwissProt: Q4KLY4 Rat

Synonyms

BAP 1 antibody

BAP1 antibody

DING antibody

DinG protein antibody

E3 ubiquitin protein ligase RING 2 antibody

E3 ubiquitin protein ligase RING2 antibody

E3 ubiquitin-protein ligase RING2 antibody

HIP2 interacting protein 3 antibody

HIP2-interacting protein 3 antibody

HIPI 3 antibody

Expand

Images

  • Western blot analysis of RING2 on different lysates with Rabbit anti-RING2 antibody (<a href="/products/ET7107-07" style="font-weight: bold;text-decoration: underline;">ET7107-07</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: COS-1 cell lysate<br />Lane 3: NIH/3T3 cell lysate<br />Lane 4: RAW264.7 cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 38 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 13 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-07" style="font-weight: bold;text-decoration: underline;">ET7107-07</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RING2 on different lysates with Rabbit anti-RING2 antibody (ET7107-07) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: COS-1 cell lysate
    Lane 3: NIH/3T3 cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: C6 cell lysate
    Lane 6: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 38 kDa
    Observed band size: 40 kDa

    Exposure time: 13 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-07) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-RING2 antibody (<a href="/products/ET7107-07" style="font-weight: bold;text-decoration: underline;">ET7107-07</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-07" style="font-weight: bold;text-decoration: underline;">ET7107-07</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-RING2 antibody (ET7107-07) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-07) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-RING2 antibody (<a href="/products/ET7107-07" style="font-weight: bold;text-decoration: underline;">ET7107-07</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-07" style="font-weight: bold;text-decoration: underline;">ET7107-07</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-RING2 antibody (ET7107-07) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-07) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-RING2 antibody (<a href="/products/ET7107-07" style="font-weight: bold;text-decoration: underline;">ET7107-07</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-07" style="font-weight: bold;text-decoration: underline;">ET7107-07</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-RING2 antibody (ET7107-07) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-07) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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