Extracellular Matrix Dynamics Antibody Sampler Kit (HAK21062)
Catalog# HAK21062
Reacts with
-
Human
Western blot analysis of Fibronectin on different lysates with Rabbit anti-Fibronectin antibody (ET1702-25) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: Caco-2 cell lysate
Lane 3: SK-MEL-28 cell lysate
Lane 4: Human kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 272 kDa
Observed band size: 272 kDa
Exposure time: 3 minutes 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-25) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: Caco-2 cell lysate
Lane 3: SK-MEL-28 cell lysate
Lane 4: Human kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 272 kDa
Observed band size: 272 kDa
Exposure time: 3 minutes 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-25) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.