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SPARC Recombinant Rabbit Monoclonal Antibody [PSH06-23]

Usd: 385

Specification

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Catalog# HA722655

SPARC Recombinant Rabbit Monoclonal Antibody [PSH06-23]

Application

  • WB

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

SPARC Recombinant Rabbit Monoclonal Antibody [PSH06-23]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SPARC aa 1-303.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 35 kDa

Positive Control

A375 cell lysate, SH-SY5Y cell lysate, MG-63 cell lysate, RWPE-1 cell lysate.

Conjugation

unconjugated

Clone Number

PSH06-23

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Osteonectin (ON) also known as secreted protein acidic and rich in cysteine (SPARC) or basement-membrane protein 40 (BM-40) is a protein that in humans is encoded by the SPARC gene. Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over-expression and ampullary cancers and chronic pancreatitis has been found.

Background References

1. Ryu S et al. The matricellular protein SPARC induces inflammatory interferon-response in macrophages during aging. Immunity. 2022 Sep

2. Veith C et al. SPARC, a Novel Regulator of Vascular Cell Function in Pulmonary Hypertension. Circulation. 2022 Mar

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

UNIPROT

SwissProt: P09486 Human

Synonyms

AA517111 antibody

Basement membrane protein 40 antibody

Basement-membrane protein 40 antibody

BM 40 antibody

BM-40 antibody

BM40 antibody

Cysteine rich protein antibody

hm:zeh0062 antibody

MGC128090 antibody

OI17 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of SPARC on different lysates with Rabbit anti-SPARC antibody (<a href="/products/HA722655" style="font-weight: bold;text-decoration: underline;">HA722655</a>) at 1/1,000 dilution.<br /><br />Lane 1: A375 cell lysate<br />Lane 2: SH-SY5Y cell lysate<br />Lane 3: MG-63 cell lysate<br />Lane 4: RWPE-1 cell lysate<br />Lane 5: MCF7 cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722655" style="font-weight: bold;text-decoration: underline;">HA722655</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of SPARC on different lysates with Rabbit anti-SPARC antibody (HA722655) at 1/1,000 dilution.

    Lane 1: A375 cell lysate
    Lane 2: SH-SY5Y cell lysate
    Lane 3: MG-63 cell lysate
    Lane 4: RWPE-1 cell lysate
    Lane 5: MCF7 cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722655) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"