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ACSL1 Mouse Monoclonal Antibody [A9B5]

Usd: 350

Specification

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Catalog# HA601112

ACSL1 Mouse Monoclonal Antibody [A9B5]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ACSL1 Mouse Monoclonal Antibody [A9B5]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human aa 151-350 / 698.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 78 kDa

Positive Control

Rat kidney tissue, human liver tissue, mouse heart tissue, Raji cell lysate, HepG2 cell lysate, LO2 cell lysate, A431 cell lysate.

Conjugation

unconjugated

Clone Number

A9B5

RRID

AB_3071834

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Long-chain-fatty-acid—CoA ligase 1 is an enzyme that in humans is encoded by the ACSL1 gene. The ACSL1 gene is located on the 4th chromosome, with its specific location being 4q35.1. The gene contains 28 exons. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. In melanocytic cells ACSL1 gene expression may be regulated by MITF. ACSL1 is known to be involved in fatty-acid metabolism critical for heart function and nonspecific mental retardation. Since the ACSL4 gene is highly expressed in brain, where it encodes a brain specific isoform, an ASCL1 mutation may be an efficient diagnostic tool in mentally retarded males.

Background References

1. Beatty A et al. Ferroptotic cell death triggered by conjugated linolenic acids is mediated by ACSL1. Nat Commun. 2021 Apr

2. Li T et al. ACSL1 affects Triglyceride Levels through the PPARγ Pathway. Int J Med Sci. 2020 Feb

Subcellular Location

Mitochondrion outer membrane, Peroxisome membrane, Microsome membrane, Endoplasmic reticulum membrane.

UNIPROT

SwissProt: P33121 Human

SwissProt: P41216 Mouse

SwissProt: P18163 Rat

Synonyms

ACS 1 antibody

ACS1 antibody

ACSL 1 antibody

ACSL1 antibody

ACSL1_HUMAN antibody

Acyl CoA synthetase 1 antibody

Acyl CoA synthetase long chain family member 1 antibody

Acyl-CoA synthetase 1 antibody

FACL 1 antibody

FACL 2 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ACSL1 on different lysates with Mouse anti-ACSL1 antibody (<a href="/products/HA601112" style="font-weight: bold;text-decoration: underline;">HA601112</a>) at 1/5,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si ACSL1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 78 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 2 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601112" style="font-weight: bold;text-decoration: underline;">HA601112</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ACSL1 on different lysates with Mouse anti-ACSL1 antibody (HA601112) at 1/5,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si ACSL1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 78 kDa
    Observed band size: 70 kDa

    Exposure time: 2 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601112) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ACSL1 on different lysates with Mouse anti-ACSL1 antibody (<a href="/products/HA601112" style="font-weight: bold;text-decoration: underline;">HA601112</a>) at 1/1,000 dilution.<br /><br />Lane 1: Raji cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: LO2 cell lysate<br />Lane 4: A431 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 78 kDa<br />Observed band size: 78 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601112" style="font-weight: bold;text-decoration: underline;">HA601112</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:150,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ACSL1 on different lysates with Mouse anti-ACSL1 antibody (HA601112) at 1/1,000 dilution.

    Lane 1: Raji cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: LO2 cell lysate
    Lane 4: A431 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 78 kDa
    Observed band size: 78 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601112) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-ACSL1 antibody (<a href="/products/HA601112" style="font-weight: bold;text-decoration: underline;">HA601112</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601112" style="font-weight: bold;text-decoration: underline;">HA601112</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-ACSL1 antibody (HA601112) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601112) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL1 antibody (<a href="/products/HA601112" style="font-weight: bold;text-decoration: underline;">HA601112</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601112" style="font-weight: bold;text-decoration: underline;">HA601112</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL1 antibody (HA601112) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601112) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Mouse anti-ACSL1 antibody (<a href="/products/HA601112" style="font-weight: bold;text-decoration: underline;">HA601112</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601112" style="font-weight: bold;text-decoration: underline;">HA601112</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Mouse anti-ACSL1 antibody (HA601112) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601112) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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metafield image

ACSL1 Rabbit Polyclonal Antibody

Application: WB

Reactivity: Human,Rat,Mouse

Conjugate: unconjugated