NGF beta Mouse Monoclonal Antibody [1G4]
Catalog# RT1428
NGF beta Mouse Monoclonal Antibody [1G4]
-
WB
-
IP
-
IF
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
NGF beta Mouse Monoclonal Antibody [1G4]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Amino acids 122-149 at the N-terminus of mature NGF of human origin.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP, IF
Molecular Weight
27kDa
Positive Control
Mouse brain tissue.
Conjugation
unconjugated
Clone Number
1G4
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃
Storage Buffer
1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:100-1:1,000
-
IP
-
1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
-
IF
-
1:50-500
Target
Function
Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds both BDNF and NT-4 equally well, and binds NT-3 to a lesser extent, while the Trk C receptor only binds NT-3.
Background References
1. von Boyen, G.B., et al. 2006. Nerve growth factor secretion in cultured enteric glia cells is modulated by proinflammatory cytokines. J. Neuroendocrinol. 18: 820-825.
2. G tz, R. and Schartl, M. 1994. The conservation of neurotrophic factors during vertebrate evolution. Comp. Biochem. Physiol. 108C: 1-10.
3. Klein, R. 1994. Role of neurotrophins in mouse neuronal development. FASEB J. 8: 738-744.
Subcellular Location
Secreted
UNIPROT
Synonyms
Beta-nerve growth factor antibody
Beta-NGF antibody
NGF antibody
NGF_HUMAN antibody
NGFB antibody
Precursor nerve growth factor antibody
proNGF antibody
proNGF_HUMAN antibody
proNGFB antibody
Images
-
Western blot analysis of NGF expression in mouse brain tissue extract.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
