CD43 Recombinant Rabbit Monoclonal Antibody [PD01-28]

Function

Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4+ T-cells and to a lesser extent by CD8+ T-cells. Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR. Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity).CD43 cytoplasmic tail: Protects cells from apoptotic signals, promoting cell survival. CD43 is a transmembrane protein involved in immune function and T-cell activation. Anti-CD43 reactivity is seen in T lymphocytes, monocytes, and granulocytes. No reactivity has been observed in normal or reactive B-cells. Reportedly, anti-CD43 reactivity is seen in the majority of T-cell lymphomas and some low grade B-cell lymphomas. Therefore, anti-CD43 is a useful immunohistochemical marker for the identification of T-cell lymphomas and some low grade B-cell lymphomas.

Background References

1. Ramirez-Pliego O et al. CD43 signals induce type one lineage commitment of human CD4+ T cells. BMC Immunol. 8:30-30(2007)

2. Galindo-Albarran A.O et al. CD43 signals prepare human T cells to receive cytokine differentiation signals. J. Cell. Physiol. 229:172-180(2014)

Tissue Specificity

Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Post-translational Modification

Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.; Phosphorylation at Ser-355 is regulated by chemokines, requires its association with ERM proteins (EZR, RDX and MSN) and is essential for its function in the regulation of T-cell trafficking to lymph nodes.; Has a high content of sialic acid and O-linked carbohydrate structures.; Cleavage by CTSG releases its extracellular domain and triggers its intramembrane proteolysis by gamma-secretase releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus.; [CD43 cytoplasmic tail]: Sumoylated.

Subcellular Location

Membrane, microvillus, uropodium, nucleus.

UNIPROT

SwissProt: P16150 Human

Synonyms