Smad4 Recombinant Rabbit Monoclonal Antibody [SP05-05] (ET1604-12)
Catalog# ET1604-12
Rabbit Monoclonal to Smad4
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-12) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Smad4 Recombinant Rabbit Monoclonal Antibody [SP05-05] (ET1604-12)
Immunogen
Synthetic peptide within c-terminal human smad4.
Host
Rabbit
Positive Control
HeLa cell lysate, HCT 116 cell lysate, C6 cell lysate, Neuro-2a cell lysates, HeLa, Neuro-2a, PC-12, human colon tissue, human lung tissue, mouse lung tissue, rat lung tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SP05-05
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:100
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 60 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Smad4
SYNONYMS
(Small) Mothers Against Decapentaplegic antibody;Deleted in Pancreatic Carcinoma 4 antibody;Deleted in Pancreatic Carcinoma antibody;Deleted in pancreatic carcinoma locus 4 antibody;Deletion target in pancreatic carcinoma 4 antibody;DPC 4 antibody;DPC4 antibody;hSMAD4 antibody;JIP antibody;MAD homolog 4 antibody;MAD mothers against decapentaplegic Drosophila homolog 4 antibody;MAD mothers against decapentaplegic homolog 4 antibody;MADH 4 antibody;MADH4 antibody;Med antibody;Medea antibody;Mothers against decapentaplegic homolog 4 antibody;Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody;Mothers against DPP homolog 4 antibody;MYHRS antibody;OTTHUMP00000163548 antibody;SMA- and MAD-related protein 4 antibody;SMAD 4 antibody;SMAD family member 4 antibody;SMAD mothers against DPP homolog 4 antibody;SMAD4 antibody;SMAD4_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the dwarfin/SMAD family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by PDPK1.; Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiquitination by USP9X restores its competence to mediate TGF-beta signaling.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.
CITATIONS
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Effect of fecal microbiota transplantation on the TGF-β1/Smad signaling pathway in rats with TNBS-induced colitis
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WB
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Rat
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Citation
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FHL3 promotes pancreatic cancer invasion and metastasis through preventing the ubiquitination degradation of EMT associated transcription factors
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WB
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human
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Citation
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Li, Z., Yin, L., Li, Y., Cao, Y., & Zeng, H.
Single-Cell RNA-Sequencing Reveals the Cellular and Genetic Heterogeneity of Skin Scar to Verify the Therapeutic Effects and Mechanism of Action of Dispel-Scar Ointment in Hypertrophic Scar Inhibition
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IF
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WB
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Rat
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Citation
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Nd: YAG laser activates the TGFBR1‐SMAD4‐Col3a1 axis via miR‐27a‐3p in photoaged rat skin
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IHC
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WB
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Citation
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