V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01] (ET1605-41)
Catalog# ET1605-41
Rabbit Monoclonal to V5 tag
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WB
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IF-Cell
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IP
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Species independent
Lane 1: 293T cell lysate
Lane 2: 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate
Lane 3: 293T transfected with V5-tagged Nanos homolog 3 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-41) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01] (ET1605-41)
Immunogen
Synthetic peptide immune sequence is cgkpipnpllgldst.
Host
Rabbit
Positive Control
V5 tagged recombinant protein.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY30-01
RRID
APPLICATION DILUTION
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WB
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1:20,000-1:50,000
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IF-Cell
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1:2,000
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
V5 tag
SYNONYMS
GKPIPNPLLGLDST epitope tag antibody;GKPIPNPLLGLDST tag antibody;Protein Rev antibody;Regulator of expression of viral proteins antibody;rev antibody;V5 epitope tag antibody
FUNCTION
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The anti-V5 antibody detects recombinant proteins containing the 14 amino acid GKPIPNPLLGLDST sequence.
CITATIONS
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Inhibition of the deubiquitinase USP9x induces pre-B cell homeobox 1 (PBX1) degradation and thereby stimulates prostate cancer cell apoptosis.
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IP
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IB
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WB
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Human
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Citation
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