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WLS Mouse Monoclonal Antibody [A7C2]

Usd: 350

Specification

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Catalog# HA600060

WLS Mouse Monoclonal Antibody [A7C2]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

WLS Mouse Monoclonal Antibody [A7C2]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human WLS aa 50-200/541.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 62 kDa

Positive Control

SH-SY5Y cell lysate, Hela cell lysate, mouse lung tissue lysate, rat brain tissue lysate, Hela, SH-SY5Y.

Conjugation

unconjugated

Clone Number

A7C2

RRID

AB_3071677

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000-1:20,000

  • IF-Cell

  • 1:100

Target

Function

Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity).

Background References

1. Zheng D. et. al. Wntless (Wls): A Prognostic Index for Progression and Patient Survival of Breast Cancer. Onco Targets Ther. 2020 Dec

2. Cheng J. et. al. Conditional deletion of Wntless in granulosa cells causes impaired corpora lutea formation and subfertility. Aging (Albany NY). 2020 Dec

Subcellular Location

Golgi apparatus membrane, Golgi apparatus membrane, Early endosome membrane, Endoplasmic reticulum membrane, Cell membrane, Cytoplasmic vesicle membrane.

UNIPROT

SwissProt: Q5T9L3 Human

SwissProt: Q6DID7 Mouse

SwissProt: Q6P689 Rat

Synonyms

C1orf139 antibody

DKFZp686I0788 antibody

Evenness interrupted, Drosophila, homolg of antibody

EVI antibody

FLJ23091 antibody

G protein coupled receptor 177 antibody

GPR 177 antibody

Integral membrane protein GPR177 antibody

MGC131760 antibody

MGC14878 antibody

Expand

Images

  • Western blot analysis of WLS on different lysates with Mouse anti-WLS antibody (<a href="/products/HA600060" style="font-weight: bold;text-decoration: underline;">HA600060</a>) at 1/20,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: Hela cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 62 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 30 seconds;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA600060" style="font-weight: bold;text-decoration: underline;">HA600060</a>) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of WLS on different lysates with Mouse anti-WLS antibody (HA600060) at 1/20,000 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: Hela cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 62 kDa
    Observed band size: 55 kDa

    Exposure time: 30 seconds;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600060) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of WLS on different lysates with Mouse anti-WLS antibody (<a href="/products/HA600060" style="font-weight: bold;text-decoration: underline;">HA600060</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse lung tissue lysate<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 62 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 30 seconds;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA600060" style="font-weight: bold;text-decoration: underline;">HA600060</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of WLS on different lysates with Mouse anti-WLS antibody (HA600060) at 1/5,000 dilution.

    Lane 1: Mouse lung tissue lysate
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 62 kDa
    Observed band size: 55 kDa

    Exposure time: 30 seconds;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600060) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Hela cells labeling WLS with Mouse anti-WLS antibody (<a href="/products/HA600060" style="font-weight: bold;text-decoration: underline;">HA600060</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-WLS antibody (<a href="/products/HA600060" style="font-weight: bold;text-decoration: underline;">HA600060</a>) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Hela cells labeling WLS with Mouse anti-WLS antibody (HA600060) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-WLS antibody (HA600060) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of SH-SY5Y cells labeling WLS with Mouse anti-WLS antibody (<a href="/products/HA600060" style="font-weight: bold;text-decoration: underline;">HA600060</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-WLS antibody (<a href="/products/HA600060" style="font-weight: bold;text-decoration: underline;">HA600060</a>) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of SH-SY5Y cells labeling WLS with Mouse anti-WLS antibody (HA600060) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-WLS antibody (HA600060) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"