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Sp7 / Osterix Recombinant Rabbit Monoclonal Antibody [PSH07-29]

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Specification

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Catalog# HA722817

Sp7 / Osterix Recombinant Rabbit Monoclonal Antibody [PSH07-29]

Application

  • WB

  • IHC-P

  • IF-Tissue

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Sp7 / Osterix Recombinant Rabbit Monoclonal Antibody [PSH07-29]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Sp7 aa 1-300.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue, IHC-Fr

Molecular Weight

Predicted band size: 45 kDa

Positive Control

Saos-2 cell lysates, human osteosarcoma tissue, mouse embryo tissue, rat embryo tissue, E14.5 mouse embryo tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH07-29

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:200-1:1,000

  • IF-Tissue

  • 1:500

  • IHC-Fr

  • 1:200

Target

Function

Transcription factor Sp7, also called osterix (Osx), is a protein that in humans is encoded by the SP7 gene. It is a member of the Sp family of zinc-finger transcription factors It is highly conserved among bone-forming vertebrate species. It plays a major role, along with Runx2 and Dlx5 in driving the differentiation of mesenchymal precursor cells into osteoblasts and eventually osteocytes. Sp7 also plays a regulatory role by inhibiting chondrocyte differentiation maintaining the balance between differentiation of mesenchymal precursor cells into ossified bone or cartilage. Mutations of this gene have been associated with multiple dysfunctional bone phenotypes in vertebrates. During development, a mouse embryo model with Sp7 expression knocked out had no formation of bone tissue. Through the use of GWAS studies, the Sp7 locus in humans has been strongly associated with bone mass density. In addition there is significant genetic evidence for its role in diseases such as Osteogenesis imperfecta (OI).

Background References

1. Wang JS et al. SP7: from Bone Development to Skeletal Disease. Curr Osteoporos Rep. 2023 Apr

2. Hojo H et al. Sp7 Action in the Skeleton: Its Mode of Action, Functions, and Relevance to Skeletal Diseases. Int J Mol Sci. 2022 May

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q8TDD2 Human

SwissProt: Q8VI67 Mouse

Entrez Gene: 300260 Rat

Synonyms

MGC126598 antibody

Osterix antibody

Osx antibody

Sp 7 antibody

SP7 antibody

Sp7 transcription factor antibody

SP7_HUMAN antibody

Transcription factor Sp7 antibody

Zinc finger protein osterix antibody

Images

  • Western blot analysis of Sp7 / Osterix on Saos-2 cell lysates (30 µg/Lane) with Rabbit anti-Sp7 / Osterix antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Sp7 / Osterix on Saos-2 cell lysates (30 µg/Lane) with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa
    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722817) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue with Rabbit anti-Sp7 / Osterix antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-Sp7 / Osterix antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>) at 1/200 dilution and competitor's antibody at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>) at 1/200 dilution and competitor's antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/200 dilution and competitor's antibody at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722817) at 1/200 dilution and competitor's antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rabbit anti-Sp7 / Osterix antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>) at 1/200 dilution and competitor's antibody at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>) at 1/200 dilution and competitor's antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/200 dilution and competitor's antibody at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722817) at 1/200 dilution and competitor's antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded E14.5 mouse embryo tissue labeling Sp7 / Osterix with Rabbit anti-Sp7 / Osterix antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>) at 1/500 dilution and competitor's antibody at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>, green) at 1/500 dilution and competitor's antibody at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded E14.5 mouse embryo tissue labeling Sp7 / Osterix with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/500 dilution and competitor's antibody at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722817, green) at 1/500 dilution and competitor's antibody at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunofluorescence analysis of frozen E14.5 mouse embryo tissue with Rabbit anti-Sp7 / Osterix antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>) at 1/200 dilution and competitor's antibody at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>, green) at 1/200 dilution and competitor's antibody at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen E14.5 mouse embryo tissue with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/200 dilution and competitor's antibody at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722817, green) at 1/200 dilution and competitor's antibody at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Western blot analysis of Sp7 / Osterix on different lysates with Rabbit anti-Sp7 / Osterix antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (no heat)<br />Lane 2: Rat brain tissue lysate (no heat)<br /><br />Notice: no heat means the lysate is not boiled. We recommend using no heat or 1% SDS hot method to prepare lysate to improve signal intensity.<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722817" style="font-weight: bold;text-decoration: underline;">HA722817</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Sp7 / Osterix on different lysates with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/1,000 dilution.

    Lane 1: Mouse brain tissue lysate (no heat)
    Lane 2: Rat brain tissue lysate (no heat)

    Notice: no heat means the lysate is not boiled. We recommend using no heat or 1% SDS hot method to prepare lysate to improve signal intensity.

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

    Exposure time: 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722817) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation