Phospho-Histone H3.3 (S31) Recombinant Rabbit Monoclonal Antibody [JE62-92] (HA721034)
Catalog# HA721034
Rabbit Monoclonal to Phospho-Histone H3.3 (S31)
Tested for
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WB
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IF-Cell
Reacts with
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Human
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Histone H3.3 (phospho S31) on Hela cell lysates.
Lane 1: Hela cells, whole cell lysate, 10ug/lane
Lane 2/3: Hela cells treated with 100ng/ml Nocodazole for 18h, whole cell lysates, 10ug/lane
Lane 4: Hela cells treated with 100ng/ml Nocodazole for 18h, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane
All lanes :
Anti-Histone H3.3 (phospho S31) antibody (HA721034) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Blocking and diluting buffer: 5% BSA.
Exposure time: 2 minutes 34 seconds
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721034) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1: Hela cells, whole cell lysate, 10ug/lane
Lane 2/3: Hela cells treated with 100ng/ml Nocodazole for 18h, whole cell lysates, 10ug/lane
Lane 4: Hela cells treated with 100ng/ml Nocodazole for 18h, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane
All lanes :
Anti-Histone H3.3 (phospho S31) antibody (HA721034) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Blocking and diluting buffer: 5% BSA.
Exposure time: 2 minutes 34 seconds
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721034) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.