CCR7 Recombinant Rabbit Monoclonal Antibody [SR36-04] (ET1602-22)
Catalog# ET1602-22
Rabbit Monoclonal to CCR7
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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Human
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Mouse
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Rat
Lane 1/2: Wild-type Hela whole cell lysate (10 µg).
Lane 3/4: CCR7 fragment 1 knockdown Hela whole cell lysate (10 µg).
Lane 5/6: CCR7 fragment 2 knockdown Hela whole cell lysate (10 µg).
ET1602-22 was shown to specifically react with CCR7 in wild-type Hela cells. Weakened was observed when CCR7 knockdown sample was tested. Wild-type and CCR7 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1602-22, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CCR7 Recombinant Rabbit Monoclonal Antibody [SR36-04] (ET1602-22)
Immunogen
Synthetic peptide within human ccr7 aa 13-62 / 378.
Host
Rabbit
Positive Control
HDLM-2 cell lysate, Jurkat cell lysate, Daudi cell lysate, MCF7 cell lysate, mouse spleen tissue lysate, mouse pancreas tissue lysate, rat spleen tissue lysate, Raji cell lysate, EL4 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Daudi, RAW264.7, C6, human tonsil tissue, human spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SR36-04
RRID
APPLICATION DILUTION
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WB
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1:2,000-1:5,000
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IF-Cell
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1:200-1:500
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IF-Tissue
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1:200
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IHC-P
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1:200
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 43 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CCR7
SYNONYMS
BLR 2 antibody;BLR2 antibody;C C chemokine receptor type 7 antibody;C C CKR 7 antibody;C-C chemokine receptor type 7 antibody;C-C CKR-7 antibody;CC chemokine receptor 7 antibody;CC chemokine receptor type 7 antibody;CC CKR 7 antibody;CC-CKR-7 antibody;CCCKR7 antibody;CCR 7 antibody;CCR-7 antibody;Ccr7 antibody;CCR7_HUMAN antibody;CD 197 antibody;CD197 antibody;CD197 antigen antibody;CDw197 antibody;Chemokine (C C motif) receptor 7 antibody;Chemokine (C C) receptor 7 antibody;Chemokine C C motif receptor 7 antibody;Chemokine C C receptor 7 antibody;Chemokine receptor 7 antibody;Chemokine receptor 7-like protein antibody;CMKBR7 antibody;EBI 1 antibody;EBI1 antibody;Ebi1h antibody;EBV Induced G Protein Coupled Receptor 1 antibody;EBV-induced G-protein coupled receptor 1 antibody;Epstein Barr virus induced G protein coupled receptor antibody;Epstein Barr virus induced gene 1 antibody;Epstein-Barr virus-induced G-protein coupled receptor 1 antibody;EVI 1 antibody;EVI1 antibody;Lymphocyte Specific G Protein Coupled Peptide Receptor antibody;MGC108519 antibody;MIP 3 beta receptor antibody;MIP-3 beta receptor antibody;MIP3 Beta Receptor antibody
SEQUENCE SIMILARITIES
Belongs to the G-protein coupled receptor 1 family.
TISSUE SPECIFICITY
Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
C-C chemokine receptor type 7 is a protein that in humans is encoded by the CCR7 gene. Two ligands have been identified for this receptor: the chemokines (C-C motif) ligand 19 (CCL19/ELC) and (C-C motif) ligand 21 (CCL21). CCR7 has also recently been designated CD197 (cluster of differentiation 197). The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. CCR7 has been shown to stimulate dendritic cell maturation. CCR7 is also involved in homing of T cells to various secondary lymphoid organs such as lymph nodes and the spleen as well as trafficking of T cells within the spleen. Activation of Dendritic cells in peripheral tissues induces CCR7 expression on the cell's surface, which recognize CCL19 and CCL21 produced in the Lymph node and increases dendritic cell expression of co-stimulation molecules (B7), and MHC class I or MHC class II.
CITATIONS
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Liu, W., Hu, H., Shao, Z., Lv, X., Zhang, Z., Deng, X., Song, Q., Han, Y., Guo, T., Xiong, L., Wang, B., & Zhang, Y.
Characterizing the tumor microenvironment at the single-cell level reveals a novel immune evasion mechanism in osteosarcoma
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IF-Tissue
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Human
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Citation
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FSH promotes immature porcine Sertoli cell proliferation by activating the CCR7/Ras-ERK signaling axis
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WB
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Pig
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Citation
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Yang Lijun,et al
Initial IL-10 production dominates the therapy of mesenchymal stem cell scaffold in spinal cord injury
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WB
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Rat
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Citation
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Li, J., Song, J., Meng, D., Yi, Y., Zhang, T., Shu, Y., & Wu, X.
Electrospun naringin-loaded microsphere/sucrose acetate isobutyrate system promotes macrophage polarization toward M2 and facilitates osteoporotic bone defect repair
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IF
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Mouse
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Citation
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Zhao Jiang,et al
Chemokine receptor 7 contributes to T-and B-cell filtering in ageing bladder, cystitis and bladder cancer
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IHC
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WB
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Human
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Mouse
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Citation
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