CD20 Recombinant Rabbit Monoclonal Antibody [SY12-01] (ET1605-33)
Catalog# ET1605-33
Rabbit Monoclonal to CD20
-
WB
-
IHC-P
-
IF-Tissue
-
IF-Cell
-
FC
-
IP
-
Human
-
Monkey
Lane 1: Raji cell lysate
Lane 2: 293T cell lysate (negative)
Lane 3: Ramos cell lysate
Lane 4: HeLa cell lysate (negative)
Lane 5: Daudi cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-33) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD20 Recombinant Rabbit Monoclonal Antibody [SY12-01] (ET1605-33)
Immunogen
Synthetic peptide within human cd20 aa 248-297 / 297.
Host
Rabbit
Positive Control
Raji cell lysate, Ramos cell lysate, Daudi cell lysate, human tonsil tissue, human spleen tissue, B-cell lymphoma tissue, Raji.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY12-01
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
IHC-P
-
1:200
-
IF-Tissue
-
1:50
-
IF-Cell
-
1:100
-
FC
-
1:1,000
-
IP
-
1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 33 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD20
SYNONYMS
APY antibody;ATOPY antibody;B lymphocyte antigen CD20 antibody;B Lymphocyte Cell Surface Antigen B1 antibody;B-lymphocyte antigen CD20 antibody;B-lymphocyte cell-surface antigen B1 antibody;B-lymphocyte surface antigen B1 antibody;B1 antibody;Bp 35 antibody;Bp35 antibody;CD 20 antibody;CD20 antibody;CD20 antigen antibody;CD20 receptor antibody;CD20_HUMAN antibody;CVID5 antibody;Fc epsilon receptor I beta chain antibody;Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody;FCER1B antibody;High affinity immunoglobulin epsilon receptor subunit beta antibody;IgE Fc receptor subunit beta antibody;IGEL antibody;IGER antibody;IGHER antibody;LEU 16 antibody;LEU16 antibody;leukocyte surface antigen Leu 16 antibody;Leukocyte surface antigen Leu-16 antibody;Ly44 antibody;Membrane spanning 4 domains A1 antibody;Membrane spanning 4 domains subfamily A member 2 antibody;Membrane-spanning 4-domains subfamily A member 1 antibody;MS4A1 antibody;MS4A2 antibody;S7 antibody
SEQUENCE SIMILARITIES
Belongs to the MS4A family.
TISSUE SPECIFICITY
Expressed on B-cells.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
CD20 is a leukocyte surface antigen consisting of four transmembrane regions and cytoplasmic N- and C-termini. The cytoplasmic domain of CD20 contains multiple phosphorylation sites, leading to additional isoforms. CD20 is expressed primarily on B cells but has also been detected on both normal and neoplastic T cells. CD20 functions as a calcium-permeable cation channel, and it is known to accelerate the G0 to G1 progression induced by IGF-1. CD20 is activated by the IGF-1 receptor via the alpha subunits of the heterotrimeric G proteins. Activation of CD20 significantly increases DNA synthesis and is thought to involve basic helix-loop-helix leucine zipper transcription factors.
CITATIONS
-
The potential immunomodulatory roles of semaphorin 4D in human periapical lesions
-
mIHC
-
Human
-
Citation
-
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.