CDK1 Recombinant Rabbit Monoclonal Antibody [SM01-44] (ET1605-54)

UNIPROT #

P06493

PROTEIN NAME

CDK1

SYNONYMS

Cdc 2 antibody;Cdc2 antibody;CDC28A antibody;CDK 1 antibody;CDK1 antibody;CDK1_HUMAN antibody;CDKN1 antibody;CELL CYCLE CONTROLLER CDC2 antibody;Cell division control protein 2 antibody;Cell division control protein 2 homolog antibody;Cell division cycle 2 G1 to S and G2 to M antibody;Cell division protein kinase 1 antibody;Cell Divsion Cycle 2 Protein antibody;Cyclin Dependent Kinase 1 antibody;Cyclin-dependent kinase 1 antibody;DKFZp686L20222 antibody;MGC111195 antibody;p34 Cdk1 antibody;p34 protein kinase antibody;P34CDC2 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

TISSUE SPECIFICITY

Isoform 2 is found in breast cancer tissues.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Thr-161 by CAK/CDK7 activates kinase activity. Phosphorylation at Thr-14 and Tyr-15 by PKMYT1 prevents nuclear translocation. Phosphorylation at Tyr-15 by WEE1 and WEE2 inhibits the protein kinase activity and acts as a negative regulator of entry into mitosis (G2 to M transition). Phosphorylation by PKMYT1 and WEE1 takes place during mitosis to keep CDK1-cyclin-B complexes inactive until the end of G2. By the end of G2, PKMYT1 and WEE1 are inactivated, but CDC25A and CDC25B are activated. Dephosphorylation by active CDC25A and CDC25B at Thr-14 and Tyr-15, leads to CDK1 activation at the G2-M transition. Phosphorylation at Tyr-15 by WEE2 during oogenesis is required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, leading to prevent meiotic reentry. Phosphorylation by WEE2 is also required for metaphase II exit during egg activation to ensure exit from meiosis in oocytes and promote pronuclear formation. Phosphorylated at Tyr-4 by PKR/EIF2AK2 upon genotoxic stress. This phosphorylation triggers CDK1 polyubiquitination and subsequent proteolysis, thus leading to G2 arrest. In response to UV irradiation, phosphorylation at Tyr-15 by PRKCD activates the G2/M DNA damage checkpoint.; Polyubiquitinated upon genotoxic stress.

SUBCELLULAR LOCATION

Mitochondrion, centrosome, spindle, Nucleus, Cytoplasm.

FUNCTION

In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclin-dependent kinase 2 (Cdk2) p33, also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2 p34-related cyclin dependent kinases have been identified. These include Cdk3-Cdk8, PCTAIRE-1-3 and KKIALRE.