Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338] (ET1608-32)

UNIPROT #

P05787

PROTEIN NAME

Cytokeratin 8

SYNONYMS

CARD2 antibody;Cell proliferation inducing gene 46 protein antibody;Cell proliferation inducing protein 46 antibody;CK 8 antibody;CK-8 antibody;CK8 antibody;CYK8 antibody;Cytokeratin 8 antibody;Cytokeratin-8 antibody;K2C8 antibody;K2C8_HUMAN antibody;K8 antibody;Keratin 8 antibody;Keratin antibody;keratin type II cytoskeletal 8 antibody;Keratin-8 antibody;KRT8 antibody;type II cytoskeletal 8 antibody;Type-II keratin Kb8 antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.; Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.; O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.

SUBCELLULAR LOCATION

Nucleoplasm, Nucleus matrix, Cytoplasm.

FUNCTION

Keratin, type II cytoskeletal 8 also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that is encoded in humans by the KRT8 gene. It is often paired with keratin 18. Antibodies to CK8 can be used to differentiate lobular carcinoma of the breast from ductal carcinoma of the breast. In normal tissue, it reacts mainly with secretory epithelia, but not with squamous epithelium, such as that found in the skin, cervix, and esophagus. However, it also reacts with a range of malignant cells, including those derived from secretory epithelia, but also some squamous carcinomata, such as spindle cell carcinoma. It is considered useful in identifying microscopic metastases of breast carcinoma in lymph nodes, and in distinguishing Paget's disease from malignant melanoma. It also reacts with neuroendocrine tumors. Keratin 8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.