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Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86]

Usd: 385

Specification

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Catalog# ET1602-44

Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86]

Application

  • WB

  • IF-Cell

  • IF-Tissue

Reactivity

  • Firefly

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Firefly Luciferase aa 132-243 / 550.

Species Reactivity

Firefly

Validated Applications

WB, IF-Cell, IF-Tissue

Molecular Weight

Predicted band size: 61 kDa

Positive Control

Luciferase recombinant protein.

Conjugation

unconjugated

Clone Number

SR03-86

RRID

AB_3069657

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Background References

1. Li D et al. An Ebola Virus-Like Particle-Based Reporter System Enables Evaluation of Antiviral Drugs In Vivo under Non-Biosafety Level 4 Conditions. J Virol 90:8720-8 (2016).

2. Simonyan H et al. A synthetic luciferin improves in vivo bioluminescence imaging of gene expression in cardiovascular brain regions. Physiol Genomics 48:762-770 (2016).

Sequence Similarity

Belongs to the ATP-dependent AMP-binding enzyme family.

Subcellular Location

Peroxisome.

UNIPROT

SwissProt: P08659 Firefly

Synonyms

ec 1.13.12.7 antibody

Firefly antibody

Luciferase antibody

Luciferin 4 monooxygenase antibody

Images

  • Western blot analysis of Firefly Luciferase on Firefly Luciferase recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1602-44" style="font-weight: bold;text-decoration: underline;">ET1602-44</a>, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Firefly Luciferase on Firefly Luciferase recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-44, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation