Hexokinase 1 Recombinant Rabbit Monoclonal Antibody [ST47-05] (ET1609-28)
Catalog# ET1609-28
Rabbit Monoclonal to Hexokinase 1
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
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Mouse
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Rat
Lane 1: MCF7 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: C2C12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 60 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Hexokinase 1 Recombinant Rabbit Monoclonal Antibody [ST47-05] (ET1609-28)
Immunogen
Synthetic peptide within human hexokinase 1 aa 100-140.
Host
Rabbit
Positive Control
MCF7 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Hela cell lysate, 293 cell lysate, HepG2 cell lysate, C2C12, MCF7, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
ST47-05
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:100-1:500
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IF-Tissue
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1:200
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IHC-P
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1:1,000
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 102 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Hexokinase 1
SYNONYMS
BB404130 antibody;Brain form hexokinase antibody;dea antibody;DrHXK1 antibody;EC 2.7.1.1 antibody;Glycolytic enzyme antibody;HEXOKIN antibody;hexokinase I antibody;Hexokinase PI antibody;Hexokinase type I antibody;Hexokinase, tumor isozyme antibody;Hexokinase-1 antibody;Hexokinase-A antibody;HK I antibody;HK1 antibody;HK1 tb antibody;Hk1-s antibody;HK1-ta antibody;HK1-tb antibody;HK1-tc antibody;HKD antibody;HKI antibody;HMSNR antibody;HXK1 antibody;HXK1_HUMAN antibody;im:7148527 antibody;mHk1-s antibody;wu:fc09d08 antibody;wu:fc16e02 antibody;wu:fc21e02 antibody;wu:fq14b11 antibody;zgc:55790 antibody;zgc:77618 antibody
SEQUENCE SIMILARITIES
Belongs to the hexokinase family.
TISSUE SPECIFICITY
Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific. Isoform 4: Testis-specific.
SUBCELLULAR LOCATION
Mitochondrion outer membrane, Cytoplasm, Membrane, Mitochondrion.
FUNCTION
The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the “set point” for insulin secretion.
CITATIONS
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Gao, F., Liang, T., Lu, Y. W., Fu, X., Dong, X., Pu, L., Hong, T., Zhou, Y., Zhang, Y., Liu, N., Zhang, F., Liu, J., Malizia, A. P., Yu, H., Zhu, W., Cowan, D. B., Chen, H., Hu, X., Mably, J. D., Wang, J., … Chen, J.
A defect in mitochondrial protein translation influences mitonuclear communication in the heart
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WB
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Mouse
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Citation
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