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LGR5 Recombinant Rabbit Monoclonal Antibody [SU32-04]

Usd: 385

Specification

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Catalog# ET1608-18

LGR5 Recombinant Rabbit Monoclonal Antibody [SU32-04]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

LGR5 Recombinant Rabbit Monoclonal Antibody [SU32-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human LGR5 aa 816-865 / 907.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 100 kDa

Positive Control

Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, mouse spinal cord tissue lysate, human skeletal muscle tissue lysate, rat kidney tissue lysate, rat spinal cord tissue lysate, SH-SY5Y.

Conjugation

unconjugated

Clone Number

SU32-04

RRID

AB_3069790

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • Use at an assay dependent concentration.

Target

Function

Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) also known as G-protein coupled receptor 49 (GPR49) or G-protein coupled receptor 67 (GPR67) is a protein that in humans is encoded by the LGR5 gene. It is a member of GPCR class A receptor proteins. R-spondin proteins are the biological ligands of LGR5. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues. LGR5 is a member of the Wnt signaling pathway. Although its ligand remains elusive, it has been shown that costimulation with R-spondin 1 and Wnt-3a induce increased internalization of LGR5. LGR5 also cointernalizes with LRP6 and FZD5 via a clathrin-dependent pathway to form a ternary complex upon Wnt ligand binding. Moreover, the rapid cointernalization of LRP6 by LGR5 induces faster rates of degradation for the former. LGR5 is crucial during embryogenesis as LGR null studies in mice incurred 100% neonatal mortality accompanied by several craniofacial distortions such as ankyloglossia and gastrointestinal dilation.

Background References

1. Kantara C et al. Methods for detecting circulating cancer stem cells (CCSCs) as a novel approach for diagnosis of colon cancer relapse/metastasis. Lab Invest 95:100-12 (2015).

2. Oshima N et al. Induction of cancer stem cell properties in colon cancer cells by defined factors. PLoS One 9:e101735 (2014).

Sequence Similarity

Belongs to the G-protein coupled receptor 1 family.

Tissue Specificity

Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.

Subcellular Location

Cell membrane, Golgi apparatus, Membrane.

UNIPROT

SwissProt: O75473 Human

SwissProt: Q9Z1P4 Mouse

Unigene:214063 Rat

Synonyms

FEX antibody

G protein coupled receptor 49 antibody

G protein coupled receptor 67 antibody

g protein-coupled receptor fex antibody

G-protein coupled receptor 49 antibody

G-protein coupled receptor 67 antibody

G-protein coupled receptor HG38 antibody

GPR 49 antibody

GPR 67 antibody

GPR49 antibody

Expand

Images

  • Western blot analysis of LGR5 on different lysates with Rabbit anti-LGR5 antibody (<a href="/products/ET1608-18" style="font-weight: bold;text-decoration: underline;">ET1608-18</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse skeletal muscle tissue lysate<br />Lane 2: Rat skeletal muscle tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 100 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-18" style="font-weight: bold;text-decoration: underline;">ET1608-18</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LGR5 on different lysates with Rabbit anti-LGR5 antibody (ET1608-18) at 1/5,000 dilution.

    Lane 1: Mouse skeletal muscle tissue lysate
    Lane 2: Rat skeletal muscle tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 100 kDa
    Observed band size: 100 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-18) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of LGR5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-18" style="font-weight: bold;text-decoration: underline;">ET1608-18</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Mouse spinal cord tissue lysate<br />Lane 2: Human skeletal muscle tissue lysate<br /><br />Predicted band size: 100 kDa<br />Observed band size: 100 kDa

    Western blot analysis of LGR5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse spinal cord tissue lysate
    Lane 2: Human skeletal muscle tissue lysate

    Predicted band size: 100 kDa
    Observed band size: 100 kDa

  • Western blot analysis of LGR5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-18" style="font-weight: bold;text-decoration: underline;">ET1608-18</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Rat skeletal muscle tissue lysate<br />Lane 2: Rat kidney tissue lysate<br />Lane 3: Rat spinal cord tissue lysate<br /><br />Predicted band size: 100 kDa<br />Observed band size: 100 kDa

    Western blot analysis of LGR5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Rat skeletal muscle tissue lysate
    Lane 2: Rat kidney tissue lysate
    Lane 3: Rat spinal cord tissue lysate

    Predicted band size: 100 kDa
    Observed band size: 100 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation