NADPH oxidase 4/NOX4 Recombinant Rabbit Monoclonal Antibody [SY0214] (ET1607-4)
Catalog# ET1607-4
Rabbit Monoclonal to NADPH oxidase 4/NOX4
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WB
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IF-Cell
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IHC-P
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IP
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FC
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Human
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Rat
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Mouse
Lane 1: SH-SY5Y cell lysate
Lane 2: U-2 OS cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
NADPH oxidase 4/NOX4 Recombinant Rabbit Monoclonal Antibody [SY0214] (ET1607-4)
Immunogen
Recombinant protein within human nox4 aa 460-578 / 578.
Host
Rabbit
Positive Control
SH-SY5Y cell lysate, U-2 OS cell lysate, SH-SY5Y, human lung tissue, human kidney tissue, rat stomach tissue, rat kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY0214
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:100
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IHC-P
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1:50-1:200
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IP
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1-2μg/sample
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 67 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
NADPH oxidase 4/NOX4
SYNONYMS
Kidney oxidase-1 antibody;Kidney superoxide-producing NADPH oxidase antibody;KOX 1 antibody;KOX antibody;Kox-1 antibody;NADPH antibody;NADPH oxidase 4 antibody;Nox4 antibody;NOX4_HUMAN antibody;Renal NAD(P)H-oxidase antibody;RENOX antibody
TISSUE SPECIFICITY
Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.
DEVELOPMENTAL STAGE
Expressed in fetal kidney and fetal liver.
POST-TRANSLATIONAL MODIFICATION
Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.
SUBCELLULAR LOCATION
Cell membrane, Nucleus, Endoplasmic reticulum membrane.
FUNCTION
The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Nox4 (also known as Renox) is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of Angiotensin II increases Nox4 expression, which suggests a role for Nox4 in vascular oxidative stress response. The gene encoding human Nox4 maps to chromosome 11q14.2-q21.
CITATIONS
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Rui Zhang,et al
DNA hypomethylation of Syk induces oxidative stressand apoptosis via the PKCβ/P66shc signaling pathway indiabetic kidney disease
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WB
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Human
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Citation
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Xu, H., Fang, L., Zeng, Q., Chen, J., Ling, H., Xia, H., Ge, Q., Wu, C., Zou, K., Wang, X., Wang, P., Yuan, W., Dong, R., Hu, S., Xiao, L., He, B., Tong, P., & Jin, H.
Glycyrrhizic acid alters the hyperoxidative stress-induced differentiation commitment of MSCs by activating the Wnt/β-catenin pathway to prevent SONFH
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WB
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Human
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Citation
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Kairui Zhang and Bin Yu
High cholesterol inhibits tendon-related gene expressions in tendon-derived stem cells through reactive oxygen species-activated nuclear factor-κB signaling. Journal of cellular physiology, 234(10), 18017–18028.
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WB
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IHC
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Mouse
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Citation
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Lin Qiuyue,et al
Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation
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WB
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Mouse
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Citation
-
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Liu Liang,et al
Tissue-resident C1q+ macrophages exert anti-aging potential through the Sirt1 pathway
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WB
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IF
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Human
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Citation
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-
Zixiang Li,et al
Avicularin inhibits ferroptosis and improves cognitive impairments in Alzheimer's disease by modulating the NOX4/Nrf2 axis
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WB
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Mouse
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Citation
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-
Glycolysis Aids in Human Lens Epithelial Cells' Adaptation to Hypoxia
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WB
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Human
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Citation
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Characterization and expression of Rubisco activase genes in Ipomoea batatas
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WB
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Ipomoea batatas
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Citation
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Xu, H., Zeng, Q., Zou, K., Huang, H., Chen, J., Wang, P., Yuan, W., Xiao, L., Tong, P., & Jin, H.
Glucocorticoid-induced activation of NOX/ROS/NF-κB signaling in MSCs contributes to the development of GONFH
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IHC
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WB
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Human
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Rat
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Citation
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