Skip to content

Contact

(857) 353-6600

orders@huabio.com

Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06] (ET1603-14)

Price: Regular price $385.00

Unit price per 

Volume

Bulk Order Quote   |   Customization Request

Catalog# ET1603-14

Rabbit Monoclonal to Phospho-EIF2S1 (S51)

Tested for

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

  • FC

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06] (ET1603-14)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding ser51 of human eif-2a.

Host

Rabbit

Modification

Phospho

Modification Site

S51

Positive Control

HeLa treated with 50nM Calyculin A for 3 hours whole cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate, C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate, THP-1 cell lysate, C2C12 cell lysate, mouse spleen tissue, rat spleen tissue, human liver tissue, human pancreas tissue, mouse brain tissue, mouse placenta tissue, mouse pancreas tissue, human prostate carcinoma tissue, human breast carcinoma tissue, human colon carcinoma tissue, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SZ01-06

RRID

AB_3069669

APPLICATION DILUTION

  • WB

  • 1:2,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:500

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 36 kDa

Isotype

IgG

TARGET

UNIPROT #

P05198

PROTEIN NAME

Phospho-EIF2S1 (S51)

SYNONYMS

EIF 2 alpha antibody;EIF 2 antibody;EIF 2A antibody;EIF 2alpha antibody;eIF-2-alpha antibody;eIF-2A antibody;EIF-2alpha antibody;EIF2 alpha antibody;EIF2 antibody;EIF2A antibody;EIF2S1 antibody;Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody;Eukaryotic translation initiation factor 2 subunit 1 alpha antibody;Eukaryotic translation initiation factor 2 subunit 1 antibody;Eukaryotic translation initiation factor 2 subunit alpha antibody;IF2A_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the WD repeat EIF2A family.

TISSUE SPECIFICITY

Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.

SUBCELLULAR LOCATION

Stress granule, Cytoplasm.

FUNCTION

Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex. eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B. Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.

CITATIONS

  • Liang Ma;Ping Fu

    Activation of GPR120 by TUG891 ameliorated cisplatin-induced acutekidney injury via repressing ER stress and apoptosis

  • Liang Ma

    2-Methylquinazoline derivative 23BB as a highly selective histone deacetylase 6 inhibitor alleviated cisplatin-induced acute kidney injury

  • Prof. Xingxu Huang,Prof. Jiajie Hou,Prof. Qiang Xia

    Intracellular XBP1-IL-24 axis dismantles cytotoxic unfolded protein response in the liver

  • Su Z

    Retinoic acid receptor-related orphan receptor α stimulates adipose tissue inflammation by modulating endoplasmic reticulum stress.

  • Wu, H., Guo, H., Liu, H., Cui, H., Fang, J., Zuo, Z., Deng, J., Li, Y., Wang, X., & Zhao, L.

    Copper sulfate-induced endoplasmic reticulum stress promotes hepatic apoptosis by activating CHOP, JNK and caspase-12 signaling pathways

  • Mao, N., Fan, Y., Liu, W., Yang, H., Yang, Y., Li, Y., Jin, F., Li, T., Yang, X., Gao, X., Cai, W., Liu, H., Xu, H., Li, S., & Yang, F.

    Oxamate Attenuates Glycolysis and ER Stress in Silicotic Mice

  • Li, Y., Cai, W., Jin, F., Wang, X., Liu, W., Li, T., Yang, X., Liu, H., Xu, H., & Yang, F.

    Thalidomide Alleviates Pulmonary Fibrosis Induced by Silica in Mice by Inhibiting ER Stress and the TLR4-NF-κB Pathway

  • Copper induce zebrafish retinal developmental defects via triggering stresses and apoptosis

  • Liang Ma,Ping Fu

    Pharmacological and genetic inhibition of fatty acid‐binding protein 4 alleviated cisplatin‐induced acute kidney injury

  • Luo, H., Shen, Y., Liao, W., Li, Q., Wu, N., Zhong, J., Xiao, C., Gan, J., Yang, Y., Dong, E., Zhang, G., Liu, B., Yue, X., Xu, L., Liu, Y., Zhao, C., Zhong, Q., & Yang, H.

    The inhibition of protein translation promotes tumor angiogenic switch

  • Qu Jiayuan,et al

    Polystyrene microplastics trigger testosterone decline via GPX1

  • Cao, X., Shu, Y., Chen, Y., Xu, Q., Guo, G., Wu, Z., Shao, M., Zhou, Y., Chen, M., Gong, Y., Li, C., Shi, Y., & Bu, H.

    Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis. Cellular and molecular gastroenterology and hepatology, S2352-345X(21)00072-2. Advance online publication.

  • Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice

  • Aerobic exercise improves non-alcoholic fatty liver disease by down-regulating the protein expression of the CNPY2-PERK pathway

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

Search

Main menu

Your cart is currently empty.
Shop now