Smad5 Recombinant Rabbit Monoclonal Antibody [SI17-06] (ET1606-26)
Catalog# ET1606-26
Rabbit Monoclonal to Smad5
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Smad5 Recombinant Rabbit Monoclonal Antibody [SI17-06] (ET1606-26)
Immunogen
Synthetic peptide within human smad5 aa 231-282 / 465.
Host
Rabbit
Positive Control
PC-3M cell lysates, Hela, A549, PC-3M, human lung tissue, mouse lung tissue, mouse testis tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SI17-06
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
52 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Smad5
SYNONYMS
DKFZp781C1895 antibody;DKFZp781O1323 antibody;Dwfc antibody;hSmad5 antibody;JV5 1 antibody;JV5-1 antibody;MAD homolog 5 antibody;MAD, mothers against decapentaplegic homolog 5 antibody;MADH 5 antibody;MADH5 antibody;Mothers against decapentaplegic homolog 5 antibody;mothers against decapentaplegic, drosophila, homolog of, 5 antibody;Mothers against DPP homolog 5 antibody;MusMLP antibody;SMA and MAD related protein 5 antibody;SMAD 5 antibody;SMAD family member 5 antibody;SMAD, mothers against DPP homolog 5 antibody;Smad5 antibody;SMAD5_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the dwarfin/SMAD family.
TISSUE SPECIFICITY
Ubiquitous.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.; Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members. Like many other TGFβ family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase. It may play a role in the pathway where TGFβ is an inhibitor of hematopoietic progenitor cells.
CITATIONS
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Cen, X., Pan, X., Zhang, B., Huang, W., Pei, F., Luo, T., Huang, X., Liu, J., & Zhao, Z.
miR-20a-5p contributes to osteogenic differentiation of human dental pulp stem cells by regulating BAMBI and activating the phosphorylation of Smad5 and p38
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WB
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Human
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Citation
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Huang, X., Pan, X., Zhang, B., Huang, W., Cen, X., Liu, J., & Zhao, Z.
CircRFWD2 Promotes Osteogenic Differentiation of human Dental Pulp Stem Cells by Targeting miR-6817-5p Through BMP-Smad and p38 MAPK Pathway
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WB
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Human
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Citation
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