5T4 Recombinant Rabbit Monoclonal Antibody [SU34-05] (ET1608-37)
Catalog# ET1608-37
Rabbit Monoclonal to 5T4
Tested for
-
WB
-
IP
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of 5T4 on different lysates with Rabbit anti-5T4 antibody (ET1608-37) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: Human lung tissue lysate (40 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 75 kDa
Exposure time: Lane 1: 25 seconds; Lane 2: 2 minutes 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: Human lung tissue lysate (40 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 75 kDa
Exposure time: Lane 1: 25 seconds; Lane 2: 2 minutes 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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