ACSL4 / FACL4 Recombinant Rabbit Monoclonal Antibody [JE56-33] - BSA and Azide free
Catalog# HA750469
ACSL4 / FACL4 Recombinant Rabbit Monoclonal Antibody [JE56-33] - BSA and Azide free
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
-
ET7111-43
含抗保成分
-
unconjugated
Overview
Product Name
ACSL4 / FACL4 Recombinant Rabbit Monoclonal Antibody [JE56-33] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human FACL4 aa 561-711 (Cytoplasmic).
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 79 kDa
Positive Control
PC-12 cell lysate, C6 cell lysate, L6 cell lysate, Rat brain tissue lysate, Rat lung tissue lysate, Rat thymus tissue lysate, A549 cell lysate, Mouse brain tissue lysate, mouse brain tissue, mouse lung tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
JE56-33
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000-1:5,000
-
IHC-P
-
1:1,000
Target
Function
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.
Background References
1. Cheng J. et. al. ACSL4 suppresses glioma cells proliferation via activating ferroptosis. Oncol Rep. 2020 Jan
2. Kuwata H. et. al. Role of acyl-CoA synthetase ACSL4 in arachidonic acid metabolism. Prostaglandins Other Lipid Mediat. 2019 Oct
Subcellular Location
Peroxisome membrane, Mitochondrion outer membrane, Microsome membrane, Endoplasmic reticulum membrane, Cell membrane.
Synonyms
ACS 4 antibody
ACS4 antibody
ACSL 4 antibody
Acsl4 antibody
ACSL4_HUMAN antibody
acyl CoA synthetase 4 antibody
Acyl CoA synthetase long chain family member 4 antibody
FACL 4 antibody
FACL4 antibody
Fatty acid Coenzyme A ligase antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/2,000 dilution.
Lane 1: Huh7 (Human liver cancer cell) cell lysate
Lane 2: Hep G2 (Human liver cancer cell) cell lysate
Lane 3: MCF7 (Human breast cancer cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Negative expression of ACSL4 / FACL4 protein in MCF7 is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA750469, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 79 kDa
Observed band size: 75 kDa -
☑ Knockdown (KD)
Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/5,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si ACSL4 / FACL4 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 79 kDa
Observed band size: 75 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750469) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/1,000 dilution.
Lane 1: PC-12 cell lysate
Lane 2: C6 cell lysate
Lane 3: L6 cell lysate
Lane 4: Rat brain tissue lysate
Lane 5: Rat lung tissue lysate
Lane 6: Rat thymus tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 79 kDa
Observed band size: 75 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750469) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/1,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 79 kDa
Observed band size: 75 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750469) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750469) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750469) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACSL4 / FACL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750469, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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