ACSL5 Recombinant Mouse Monoclonal Antibody [A9D3-R]
Catalog# HA601166
ACSL5 Recombinant Mouse Monoclonal Antibody [A9D3-R]
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WB
-
IHC-P
-
Human
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Mouse
-
Rat
-
unconjugated
Overview
Product Name
ACSL5 Recombinant Mouse Monoclonal Antibody [A9D3-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human ACSL5 aa 151-350 / 683.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 76 kDa
Positive Control
Mouse liver tissue lysate, rat liver tissue lysate, mouse white adipose tissue lysate, mouse brown adipose tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue.
Conjugation
unconjugated
Clone Number
A9D3-R
RRID
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
Target
Function
Long-chain-fatty-acid—CoA ligase 5 is an enzyme that in humans is encoded by the ACSL5 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.
Background References
1. Hou T et al. Cytoplasmic SIRT6-mediated ACSL5 deacetylation impedes nonalcoholic fatty liver disease by facilitating hepatic fatty acid oxidation. Mol Cell. 2022 Nov
2. Luo Q et al. Role of ACSL5 in fatty acid metabolism. Heliyon. 2023 Jan
Subcellular Location
Mitochondrion, Endoplasmic reticulum, Mitochondrion outer membrane, Endoplasmic reticulum membrane, Cell membrane.
Synonyms
ACS2 antibody
ACS5 antibody
Acyl CoA synthetase 5 antibody
Acyl CoA synthetase long chain family member 5 antibody
EC 6.2.1.3 antibody
FACL5 antibody
FACL5 for fatty acid coenzyme A ligase 5 antibody
Fatty acid CoA ligase, long chain 5 antibody
Fatty acid coenzyme A ligase 5 antibody
Fatty acid Coenzyme A ligase long chain 5 antibody
ExpandImages
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Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (HA601166) at 1/1,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 76 kDa
Observed band size: 65 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601166) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (HA601166) at 1/1,000 dilution.
Lane 1: Mouse white adipose tissue lysate
Lane 2: Mouse brown adipose tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 76 kDa
Observed band size: 65 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601166) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL5 antibody (HA601166) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601166) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ACSL5 antibody (HA601166) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601166) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-ACSL5 antibody (HA601166) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601166) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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