Skip to content

ADAM10 Recombinant Rabbit Monoclonal Antibody [JM32-11]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET1703-60

ADAM10 Recombinant Rabbit Monoclonal Antibody [JM32-11]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ADAM10 Recombinant Rabbit Monoclonal Antibody [JM32-11]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human ADAM10 aa 715-748 / 748.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 84 kDa

Positive Control

Jurkat cell lysate, HeLa cell lysate, human lung tissue lysate, RAW264.7 cell lysate, mouse placenta tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, rat spleen tissue lysate, human thyroid carcinoma tissue, human stomach tissue.

Conjugation

unconjugated

Clone Number

JM32-11

RRID

AB_3070416

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

ADAM (a disintegrin and metalloprotease) proteins are a family of over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases that are involved in cell-cell, cell-matrix interface related processes including fertilization, muscle fusion, secretion of TNFα (tumor necrosis factor α), and modulation of the neurogenic function of Notch and Delta. ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (integrin ligand) a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane domain and a cytoplasmic tail. ADAMs are expressed in brain, testis, epididymis, ovary, breast, placenta, liver, heart, lung, bone and muscle, and catalyze proteolysis, adhesion, fusion and intracellular signaling. ADAM10 is a TNF-processing enzyme that cleaves pro-TNF, a membrane-bound precusor protein, at Ala 76-Val 77, which causes membrane shedding of soluble TNF.

Background References

1. Yang C et al. Enhancement of the nonamyloidogenic pathway by exogenous NGF in an Alzheimer transgenic mouse model. Neuropeptides 48:233-8 (2014).

2. Guo Q et al. Adam10 Mediates the Choice between Principal Cells and Intercalated Cells in the Kidney. J Am Soc Nephrol N/A:N/A (2014).

Tissue Specificity

Expressed in the brain (at protein level). Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.

Post-translational Modification

The precursor is cleaved by furin and PCSK7.

Subcellular Location

Cell membrane. Endomembrane system.

UNIPROT

SwissProt: O14672 Human

SwissProt: O35598 Mouse

SwissProt: Q10743 Rat

Synonyms

A disintegrin and metalloprotease domain 10 antibody

A disintegrin and metalloproteinase domain 10 antibody

AD 10 antibody

AD10 antibody

AD18 antibody

ADA10_HUMAN antibody

ADAM 10 antibody

ADAM metallopeptidase domain 10 antibody

ADAM10 antibody

CD 156c antibody

Expand

Images

  • Western blot analysis of ADAM10 on different lysates with Rabbit anti-ADAM10 antibody (<a href="/products/ET1703-60" style="font-weight: bold;text-decoration: underline;">ET1703-60</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate (15 µg/Lane)<br />Lane 2: HeLa cell lysate (15 µg/Lane)<br />Lane 3: Human lung tissue lysate (30 µg/Lane)<br />Lane 4: RAW264.7 cell lysate (15 µg/Lane)<br />Lane 5: Mouse placenta tissue lysate (30 µg/Lane)<br />Lane 6: Mouse brain tissue lysate (30 µg/Lane)<br />Lane 7: Rat brain tissue lysate (30 µg/Lane)<br />Lane 8: Rat spleen tissue lysate (30 µg/Lane)<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 84 kDa<br />Observed band size: 75/100 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-60" style="font-weight: bold;text-decoration: underline;">ET1703-60</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ADAM10 on different lysates with Rabbit anti-ADAM10 antibody (ET1703-60) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate (15 µg/Lane)
    Lane 2: HeLa cell lysate (15 µg/Lane)
    Lane 3: Human lung tissue lysate (30 µg/Lane)
    Lane 4: RAW264.7 cell lysate (15 µg/Lane)
    Lane 5: Mouse placenta tissue lysate (30 µg/Lane)
    Lane 6: Mouse brain tissue lysate (30 µg/Lane)
    Lane 7: Rat brain tissue lysate (30 µg/Lane)
    Lane 8: Rat spleen tissue lysate (30 µg/Lane)

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 84 kDa
    Observed band size: 75/100 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-60) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ADAM10 on different lysates with Rabbit anti-ADAM10 antibody (<a href="/products/ET1703-60" style="font-weight: bold;text-decoration: underline;">ET1703-60</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si ADAM10 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 84 kDa<br />Observed band size: 84/70 kDa<br /><br />Exposure time: 2 minutes ; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-60" style="font-weight: bold;text-decoration: underline;">ET1703-60</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ADAM10 on different lysates with Rabbit anti-ADAM10 antibody (ET1703-60) at 1/1,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si ADAM10 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 84 kDa
    Observed band size: 84/70 kDa

    Exposure time: 2 minutes ; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-60) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-ADAM10 antibody (<a href="/products/ET1703-60" style="font-weight: bold;text-decoration: underline;">ET1703-60</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-60" style="font-weight: bold;text-decoration: underline;">ET1703-60</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-ADAM10 antibody (ET1703-60) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-60) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ADAM10 was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/ET1703-60" style="font-weight: bold;text-decoration: underline;">ET1703-60</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/ET1703-60" style="font-weight: bold;text-decoration: underline;">ET1703-60</a> at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/ET1703-60" style="font-weight: bold;text-decoration: underline;">ET1703-60</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/ET1703-60" style="font-weight: bold;text-decoration: underline;">ET1703-60</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 59 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    ADAM10 was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1703-60 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1703-60 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: ET1703-60 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of ET1703-60 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 59 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

ADAM10 Rabbit Polyclonal Antibody

Application: WB,IHC-P

Reactivity: Human

Conjugate: unconjugated