ADAMTS13 Recombinant Rabbit Monoclonal Antibody [PSH29-01]
Catalog# HA724829
ADAMTS13 Recombinant Rabbit Monoclonal Antibody [PSH29-01]
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WB
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Human
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unconjugated
Overview
Product Name
ADAMTS13 Recombinant Rabbit Monoclonal Antibody [PSH29-01]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human ADAMTS13 aa 75-350.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size 154 kDa
Positive Control
Human serum.
Conjugation
unconjugated
Clone Number
PSH29-01
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | |
|---|---|---|
| human |
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|
| mouse |
|
|
| rat |
|
|
Product Features
Form
Liquid
Concentration
0.5 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13)—also known as von Willebrand factor-cleaving protease (VWFCP)—is a zinc-containing metalloprotease enzyme that cleaves von Willebrand factor (vWf), a large protein involved in blood clotting. It is secreted in blood and degrades large vWf multimers, decreasing their activity. Glycosylated. O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL.
Background References
1. Akiyama M et al. Crystal structures of the noncatalytic domains of ADAMTS13 reveal multiple discontinuous exosites for von Willebrand factor. Proc Natl Acad Sci USA 106:19274-19279 (2009).
2. Fujikawa K et al. Purification of human von Willebrand factor-cleaving protease and its identification as a new member of the metalloproteinase family. Blood 98:1662-1666 (2001).
Subcellular Location
Secreted.
UNIPROT
Synonyms
A disintegrin and metalloproteinase with thrombospondin motifs 13 antibody
A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13 antibody
A disintegrin like and metalloprotease with thrombospondin type 1 motif 13 antibody
ADAM metallopeptidase with thrombospondin type 1 motif 13 antibody
ADAM TS antibody
ADAM-TS 13 antibody
ADAM-TS13 antibody
ADAMTS 13 antibody
ADAMTS-13 antibody
ADAMTS13 antibody
ExpandImages
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Western blot analysis of ADAMTS13 on human serum with Rabbit anti-ADAMTS13 antibody (HA724829) at 1/2,000 dilution.
Lysates/proteins at 30 µg/Lane.
Exposure time: 1 minute; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724829, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 154 kDa
Observed band size: 154 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
ADAMTS13 Recombinant Rabbit Monoclonal Antibody [JG39-21]
Application: WB,IHC-P
Reactivity: Human,Rat
Conjugate: unconjugated
