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AFP Mouse Monoclonal Antibody [A5-G3]

Usd: 350

Specification

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Catalog# EM1701-31

AFP Mouse Monoclonal Antibody [A5-G3]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

AFP Mouse Monoclonal Antibody [A5-G3]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human AFP aa 1-200 / 609.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 69 kDa

Positive Control

Human placenta tissue lysates, A549 cell lysate, Hela cell lysate, HepG2 cell lysate, human liver cancer tissue, human lung cancer tissue, human liver tissue.

Conjugation

unconjugated

Clone Number

A5-G3

RRID

AB_3068756

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

Target

Function

α-fetoprotein (AFP) is expressed in fetal liver at varying levels throughout development and is present only in trace amounts in normal adult tissues. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker. AFP binds copper, nickel and fatty acids, and in some cases may bind serum albumin or estrogen. It has been demonstrated that the AFP promoter is a target for NF-1 (nuclear factor-1), HNF-1 (hepatocyte nuclear factor-1) and C/EBP transcription factors. While HNF-1 binding to the AFP promoter results in AFP expression, NF-1 binding results in a decrease in AFP promoter activity.

Background References

1. Li Y et al. Abnormal Neural Progenitor Cells Differentiated from Induced Pluripotent Stem Cells Partially Mimicked Development of TSC2 Neurological Abnormalities. Stem Cell Reports 8:883-893 (2017).

2. Ahmadian Baghbaderani B et al. A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells. PLoS One 11:e0161229 (2016).

Sequence Similarity

Belongs to the ALB/AFP/VDB family.

Tissue Specificity

Plasma. Synthesized by the fetal liver and yolk sac.

Post-translational Modification

Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.; Sulfated.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P02771 Human

Synonyms

Afp antibody

AFPD antibody

Alpha fetoglobulin antibody

Alpha fetoprotein antibody

Alpha fetoprotein precursor antibody

Alpha-1-fetoprotein antibody

Alpha-fetoglobulin antibody

Alpha-fetoprotein antibody

alpha-fetoprotein, Hereditary persistence of, included antibody

FETA antibody

Expand

Images

  • Western blot analysis of AFP on human placenta tissue lysate using anti-AFP antibody at 1/1,000 dilution.

    Western blot analysis of AFP on human placenta tissue lysate using anti-AFP antibody at 1/1,000 dilution.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of AFP on different lysates with Mouse anti-AFP antibody (<a href="/products/EM1701-31" style="font-weight: bold;text-decoration: underline;">EM1701-31</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-WT cell lysate (10 µg/Lane)<br />Lane 2: A549-KD AFP cell lysate (10 µg/Lane)<br /><br />Predicted band size: 69 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-31" style="font-weight: bold;text-decoration: underline;">EM1701-31</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of AFP on different lysates with Mouse anti-AFP antibody (EM1701-31) at 1/1,000 dilution.

    Lane 1: A549-WT cell lysate (10 µg/Lane)
    Lane 2: A549-KD AFP cell lysate (10 µg/Lane)

    Predicted band size: 69 kDa
    Observed band size: 80 kDa

    Exposure time: 30 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of AFP on different lysates with Mouse anti-AFP antibody (<a href="/products/EM1701-31" style="font-weight: bold;text-decoration: underline;">EM1701-31</a>) at 1/500 dilution.<br /><br />Lane 1: Hela cell lysate<br />Lane 2: HepG2 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 69 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 2 minutes;<br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-31" style="font-weight: bold;text-decoration: underline;">EM1701-31</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AFP on different lysates with Mouse anti-AFP antibody (EM1701-31) at 1/500 dilution.

    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 69 kDa
    Observed band size: 80 kDa

    Exposure time: 2 minutes;
    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-31) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AFP antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AFP antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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