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AKR1C1 Mouse Monoclonal Antibody [5-F10]

Usd: 350

Specification

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Catalog# EM1701-21

AKR1C1 Mouse Monoclonal Antibody [5-F10]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

AKR1C1 Mouse Monoclonal Antibody [5-F10]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human AKR1C1 aa 180-323 / 323.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 37 kDa

Positive Control

HepG2 cell lysate, U-87 MG cell lysate, HeLa cell lysate, A431 cell lysate, human liver tissue lysate, human liver tissue, human pancreas tissue, mouse kidney tissue.

Conjugation

unconjugated

Clone Number

5-F10

RRID

AB_3068749

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

Target

Function

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH. Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens. May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate. Displays affinity for bile acids.

Background References

1. Woo S et al. AKR1C1 as a Biomarker for Differentiating the Biological Effects of Combustible from Non-Combustible Tobacco Products. Genes (Basel) 8(5) (2017).

2. Zeng CM et al. Aldo-Keto Reductase AKR1C1-AKR1C4: Functions, Regulation, and Intervention for Anti-cancer Therapy. Front Pharmacol 8:119 (2017).

Sequence Similarity

Belongs to the aldo/keto reductase family.

Tissue Specificity

Expressed in all tissues tested including liver, prostate, testis, adrenal gland, brain, uterus, mammary gland and keratinocytes. Highest levels found in liver, mammary gland and brain.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q04828 Human

Synonyms

2-dihydrobenzene-1 antibody

2-diol dehydrogenase antibody

20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody

20 ALPHA HSD antibody

20 alpha hydroxysteroid dehydrogenase antibody

20-alpha-HSD antibody

20-alpha-hydroxysteroid dehydrogenase antibody

20ALPHAHSD antibody

2ALPHAHSD antibody

AK1C1 antibody

Expand

Images

  • Western blot analysis of AKR1C1 on different lysates with Mouse anti-AKR1C1 antibody (<a href="/products/EM1701-21" style="font-weight: bold;text-decoration: underline;">EM1701-21</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: U-87 MG cell lysate (20 µg/Lane)<br />Lane 3: HeLa cell lysate (20 µg/Lane)<br />Lane 4: A431 cell lysate (20 µg/Lane)<br />Lane 5: Human liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 37 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 10 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-21" style="font-weight: bold;text-decoration: underline;">EM1701-21</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AKR1C1 on different lysates with Mouse anti-AKR1C1 antibody (EM1701-21) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: U-87 MG cell lysate (20 µg/Lane)
    Lane 3: HeLa cell lysate (20 µg/Lane)
    Lane 4: A431 cell lysate (20 µg/Lane)
    Lane 5: Human liver tissue lysate (40 µg/Lane)

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

    Exposure time: 10 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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