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ALDH2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1407-3

ALDH2 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

ALDH2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human ALDH2 aa 18-80.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 56 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, SK-Br-3 cell lysate, THP-1 cell lysate, human liver tissue lysate, rat lung tissue lysate, Hela, HepG2.

Conjugation

unconjugated

RRID

AB_3073298

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:200

Target

Function

Aldehyde dehydrogenase, mitochondrial is an enzyme that in humans is encoded by the ALDH2 gene located on chromosome 12. This protein belongs to the aldehyde dehydrogenase family of enzymes. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Mitochondrial aldehyde dehydrogenase belongs to the aldehyde dehydrogenase family of enzymes that catalyze the chemical transformation from acetaldehyde to acetic acid. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Additionally, ALDH2 functions as a protector against oxidative stress.

Background References

1. "Mitochondrial aldehyde dehydrogenase polymorphism in Asian and American Indian populations: detection of new ALDH2 alleles." Novoradovsky A., Tsai S.J., Goldfarb L., Peterson R., Long J.C., Goldman D. Alcohol. Clin. Exp. Res. 19:1105-1110(1995)

2. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome." Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H. J. Proteomics 96:253-262(2014)

Sequence Similarity

Belongs to the aldehyde dehydrogenase family.

Subcellular Location

Mitochondrion matrix.

UNIPROT

SwissProt: P05091 Human

SwissProt: P47738 Mouse

SwissProt: P11884 Rat

Synonyms

Acetaldehyde dehydrogenase 2 antibody

Aldehyde dehydrogenase 2 family (mitochondrial) antibody

Aldehyde dehydrogenase 2 family antibody

Aldehyde dehydrogenase mitochondrial antibody

Aldehyde dehydrogenase, mitochondrial antibody

ALDH 2 antibody

ALDH class 2 antibody

ALDH E2 antibody

ALDH-E2 antibody

Aldh2 antibody

Expand

Images

  • Western blot analysis of ALDH2 on different lysates with Rabbit anti-ALDH2 antibody (<a href="/products/R1407-3" style="font-weight: bold;text-decoration: underline;">R1407-3</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (15 µg/Lane)<br />Lane 2: HepG2 cell lysate (15 µg/Lane)<br />Lane 3: SK-Br-3 cell lysate (15 µg/Lane)<br />Lane 4: THP-1 cell lysate (15 µg/Lane)<br />Lane 5: Human liver tissue lysate (30 µg/Lane)<br />Lane 6: Rat lung tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 56 kDa<br />Observed band size: 56 kDa<br /><br />Exposure time: 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1407-3" style="font-weight: bold;text-decoration: underline;">R1407-3</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ALDH2 on different lysates with Rabbit anti-ALDH2 antibody (R1407-3) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (15 µg/Lane)
    Lane 2: HepG2 cell lysate (15 µg/Lane)
    Lane 3: SK-Br-3 cell lysate (15 µg/Lane)
    Lane 4: THP-1 cell lysate (15 µg/Lane)
    Lane 5: Human liver tissue lysate (30 µg/Lane)
    Lane 6: Rat lung tissue lysate (30 µg/Lane)

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

    Exposure time: 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1407-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • ICC staining ALDH2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

    ICC staining ALDH2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

  • ICC staining ALDH2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

    ICC staining ALDH2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Products with the same target and pathway

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Conjugate: unconjugated