AMH Recombinant Rabbit Monoclonal Antibody [PSH13-48] - BSA and Azide free
Catalog# HA751467
AMH Recombinant Rabbit Monoclonal Antibody [PSH13-48] - BSA and Azide free
-
WB
-
IHC-Fr
-
IHC-P
-
IF-Tissue
-
Mouse
-
Rat
-
Human
-
unconjugated
Overview
Product Name
AMH Recombinant Rabbit Monoclonal Antibody [PSH13-48] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human AMH aa 411-560.
Species Reactivity
Mouse, Rat (Predicted: Human)
Predicted species support after-sales service
Validated Applications
WB, IHC-Fr, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 59 kDa
Positive Control
Mouse ovary tissue lysate, Mouse testis tissue lysate, Rat ovary tissue lysate, mouse ovary tissue, rat ovary tissue.
Conjugation
unconjugated
Clone Number
PSH13-48
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IHC-Fr
-
1:500
-
IHC-P
-
1:500-1:2,000
-
IF-Tissue
-
1:500
Target
Function
Anti-Müllerian hormone (AMH), also known as Müllerian-inhibiting hormone (MIH), is a glycoprotein hormone structurally related to inhibin and activin from the transforming growth factor beta superfamily, whose key roles are in growth differentiation and folliculogenesis. In humans, it is encoded by the AMH gene, on chromosome 19p13.3, while its receptor is encoded by the AMHR2 gene on chromosome 12. AMH is activated by SOX9 in the Sertoli cells of the male fetus. Its expression inhibits the development of the female reproductive tract, or Müllerian ducts (paramesonephric ducts), in the male embryo, thereby arresting the development of fallopian tubes, uterus, and upper vagina. AMH expression is critical to sex differentiation at a specific time during fetal development, and appears to be tightly regulated by nuclear receptor SF-1, transcription GATA factors, sex-reversal gene DAX1, and follicle-stimulating hormone (FSH). Mutations in both the AMH gene and the type II AMH receptor have been shown to cause the persistence of Müllerian derivatives in males that are otherwise normally masculinized.
Background References
1. Cedars MI. Evaluation of Female Fertility-AMH and Ovarian Reserve Testing. J Clin Endocrinol Metab. 2022 May
2. Howard JA et al. Molecular Mechanisms of AMH Signaling. Front Endocrinol (Lausanne). 2022 Jun
Subcellular Location
Secreted.
Synonyms
Anti muellerian hormone antibody
MIF antibody
MIS antibody
Muellerian inhibiting factor antibody
Mullerian inhibiting substance antibody
Images
-
☑ Relative expression (RE)
Western blot analysis of AMH on different lysates with Rabbit anti-AMH antibody (HA751467) at 1/5,000 dilution.
Lane 1: Mouse ovary tissue lysate
Lane 2: Mouse spleen tissue lysate (negative)
Lane 3: Mouse testis tissue lysate
Lane 4: Rat ovary tissue lysate
Lane 5: Rat spleen tissue lysate (negative)
Lysates/proteins at 40 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: Lane 1-3: 42 seconds; Lane 4-5: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751467) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IHC-Fr
Species: Mouse
Site: ovary
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: ovary
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-AMH antibody (HA751467) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751467) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-AMH antibody (HA751467) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751467) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Mouse
Site: ovary
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
Application: IF-Tissue
Species: Rat
Site: ovary
Sample: Paraffin-embedded section
Antibody concentration: 1/500
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
AMH Recombinant Rabbit Monoclonal Antibody [PSH13-48]
Application: WB,IHC-Fr,IHC-P,IF-Tissue
Reactivity: Mouse,Rat,Human
Conjugate: unconjugated
