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AMH Recombinant Rabbit Monoclonal Antibody [PSH13-48] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751467

AMH Recombinant Rabbit Monoclonal Antibody [PSH13-48] - BSA and Azide free

Application

  • WB

  • IHC-Fr

  • IHC-P

  • IF-Tissue

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Human

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

AMH Recombinant Rabbit Monoclonal Antibody [PSH13-48] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human AMH aa 411-560.

Species Reactivity

Mouse, Rat (Predicted: Human)

Predicted species support after-sales service

Validated Applications

WB, IHC-Fr, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 59 kDa

Positive Control

Mouse ovary tissue lysate, Mouse testis tissue lysate, Rat ovary tissue lysate, mouse ovary tissue, rat ovary tissue.

Conjugation

unconjugated

Clone Number

PSH13-48

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:500-1:2,000

  • IF-Tissue

  • 1:500

Target

Function

Anti-Müllerian hormone (AMH), also known as Müllerian-inhibiting hormone (MIH), is a glycoprotein hormone structurally related to inhibin and activin from the transforming growth factor beta superfamily, whose key roles are in growth differentiation and folliculogenesis. In humans, it is encoded by the AMH gene, on chromosome 19p13.3, while its receptor is encoded by the AMHR2 gene on chromosome 12. AMH is activated by SOX9 in the Sertoli cells of the male fetus. Its expression inhibits the development of the female reproductive tract, or Müllerian ducts (paramesonephric ducts), in the male embryo, thereby arresting the development of fallopian tubes, uterus, and upper vagina. AMH expression is critical to sex differentiation at a specific time during fetal development, and appears to be tightly regulated by nuclear receptor SF-1, transcription GATA factors, sex-reversal gene DAX1, and follicle-stimulating hormone (FSH). Mutations in both the AMH gene and the type II AMH receptor have been shown to cause the persistence of Müllerian derivatives in males that are otherwise normally masculinized.

Background References

1. Cedars MI. Evaluation of Female Fertility-AMH and Ovarian Reserve Testing. J Clin Endocrinol Metab. 2022 May

2. Howard JA et al. Molecular Mechanisms of AMH Signaling. Front Endocrinol (Lausanne). 2022 Jun

Subcellular Location

Secreted.

UNIPROT

SwissProt: P03971 Human

SwissProt: P27106 Mouse

SwissProt: P49000 Rat

Synonyms

Anti muellerian hormone antibody

MIF antibody

MIS antibody

Muellerian inhibiting factor antibody

Mullerian inhibiting substance antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of AMH on different lysates with Rabbit anti-AMH antibody (<a href="/products/HA751467" style="font-weight: bold;text-decoration: underline;">HA751467</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse ovary tissue lysate<br />Lane 2: Mouse spleen tissue lysate (negative)<br />Lane 3: Mouse testis tissue lysate<br />Lane 4: Rat ovary tissue lysate<br />Lane 5: Rat spleen tissue lysate (negative)<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 59 kDa<br /><br />Exposure time: Lane 1-3: 42 seconds; Lane 4-5: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751467" style="font-weight: bold;text-decoration: underline;">HA751467</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of AMH on different lysates with Rabbit anti-AMH antibody (HA751467) at 1/5,000 dilution.

    Lane 1: Mouse ovary tissue lysate
    Lane 2: Mouse spleen tissue lysate (negative)
    Lane 3: Mouse testis tissue lysate
    Lane 4: Rat ovary tissue lysate
    Lane 5: Rat spleen tissue lysate (negative)

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

    Exposure time: Lane 1-3: 42 seconds; Lane 4-5: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751467) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: ovary<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: ovary

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: ovary<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: ovary

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-AMH antibody (<a href="/products/HA751467" style="font-weight: bold;text-decoration: underline;">HA751467</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751467" style="font-weight: bold;text-decoration: underline;">HA751467</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-AMH antibody (HA751467) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751467) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-AMH antibody (<a href="/products/HA751467" style="font-weight: bold;text-decoration: underline;">HA751467</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751467" style="font-weight: bold;text-decoration: underline;">HA751467</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-AMH antibody (HA751467) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751467) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: ovary<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Mouse

    Site: ovary

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

  • Application: IF-Tissue<br /><br />Species: Rat<br /><br />Site: ovary<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Rat

    Site: ovary

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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