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APC Recombinant Rabbit Monoclonal Antibody [SC61-03]

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Specification

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Catalog# ET1610-80

APC Recombinant Rabbit Monoclonal Antibody [SC61-03]

Application

  • WB

  • IP

  • IHC-P

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

APC Recombinant Rabbit Monoclonal Antibody [SC61-03]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human APC aa 51-100 / 2,843.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IP, IHC-P, IHC-Fr

Molecular Weight

Predicted band size: 312 kDa

Positive Control

Mouse brain tissue, mouse striatum tissue, rat brain tissue, rat striatum tissue, mouse brain tissue lysates, 293, A431.

Conjugation

unconjugated

Clone Number

SC61-03

RRID

AB_3069966

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000-1:20,000

  • IHC-P

  • 1:200-1:1,000

  • IHC-Fr

  • 1:500

Target

Function

Adenomatous polyposis coli (APC) also known as deleted in polyposis 2.5 (DP2.5) is a protein that in humans is encoded by the APC gene. The APC protein is a negative regulator that controls beta-catenin concentrations and interacts with E-cadherin, which are involved in cell adhesion. Mutations in the APC gene may result in colorectal cancer. APC is classified as a tumor suppressor gene. Tumor suppressor genes prevent the uncontrolled growth of cells that may result in cancerous tumors. The protein made by the APC gene plays a critical role in several cellular processes that determine whether a cell may develop into a tumor. The APC protein helps control how often a cell divides, how it attaches to other cells within a tissue, how the cell polarizes and the morphogenesis of the 3D structures, or whether a cell moves within or away from tissue. This protein also helps ensure that the chromosome number in cells produced through cell division is correct. The APC protein accomplishes these tasks mainly through association with other proteins, especially those that are involved in cell attachment and signaling. The activity of one protein in particular, beta-catenin, is controlled by the APC protein (see: Wnt signaling pathway). Regulation of beta-catenin prevents genes that stimulate cell division from being turned on too often and prevents cell overgrowth.

Background References

1. Liu R et al. Fstl1 is involved in the regulation of radial glial scaffold development. Mol Brain 8:53 (2015).

2. Parisi A et al. APC is required for muscle stem cell proliferation and skeletal muscle tissue repair. J Cell Biol 210:717-26 (2015).

Sequence Similarity

Belongs to the adenomatous polyposis coli (APC) family.

Tissue Specificity

Expressed in a variety of tissues: brain, small intestine, colon, thymus, skeletal muscle, heart, prostate, lung, spleen, ovary, testis kidney, placenta, blood and liver. Isoform 1A: Very strongly expressed in brain but has relatively low expression levels in other tissues. Isoform 1B: Predominant form in all tissues except for brain, including gastric mucosa and blood.

Post-translational Modification

Phosphorylated by GSK3B.; Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.

Subcellular Location

Cytoplasm, Cell junction, Cell membrane.

UNIPROT

SwissProt: P25054 Human

SwissProt: Q61315 Mouse

SwissProt: P70478 Rat

Synonyms

Adenomatous Polyposis Coli antibody

Adenomatous polyposis coli protein antibody

Apc antibody

APC_HUMAN antibody

CC1 antibody

Deleted in polyposis 2.5 antibody

DP2 antibody

DP2.5 antibody

DP3 antibody

FAP antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    Application: IHC-Fr

    Species: Rat

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-APC antibody (<a href="/products/ET1610-80" style="font-weight: bold;text-decoration: underline;">ET1610-80</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-80" style="font-weight: bold;text-decoration: underline;">ET1610-80</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-APC antibody (ET1610-80) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-80) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-APC antibody (<a href="/products/ET1610-80" style="font-weight: bold;text-decoration: underline;">ET1610-80</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-80" style="font-weight: bold;text-decoration: underline;">ET1610-80</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-APC antibody (ET1610-80) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-80) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-APC antibody (<a href="/products/ET1610-80" style="font-weight: bold;text-decoration: underline;">ET1610-80</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-80" style="font-weight: bold;text-decoration: underline;">ET1610-80</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-APC antibody (ET1610-80) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-80) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-APC antibody (<a href="/products/ET1610-80" style="font-weight: bold;text-decoration: underline;">ET1610-80</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-80" style="font-weight: bold;text-decoration: underline;">ET1610-80</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-APC antibody (ET1610-80) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-80) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of APC on different lysates with Rabbit anti-APC antibody (<a href="/products/ET1610-80" style="font-weight: bold;text-decoration: underline;">ET1610-80</a>) at 1/5,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: C2C12 cell lysate<br />Lane 3: C6 cell lysate<br />Lane 4: mouse brain tissue lysate<br />Lane 5: rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 160 kDa<br />Observed band size: 160 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-80" style="font-weight: bold;text-decoration: underline;">ET1610-80</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of APC on different lysates with Rabbit anti-APC antibody (ET1610-80) at 1/5,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: C2C12 cell lysate
    Lane 3: C6 cell lysate
    Lane 4: mouse brain tissue lysate
    Lane 5: rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 160 kDa
    Observed band size: 160 kDa

    Exposure time: 2 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-80) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of APC on different lysates with Rabbit anti-APC antibody (<a href="/products/ET1610-80" style="font-weight: bold;text-decoration: underline;">ET1610-80</a>) at 1/5,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: C2C12 cell lysate<br />Lane 3: C6 cell lysate<br />Lane 4: mouse brain tissue lysate<br />Lane 5: rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 160 kDa<br />Observed band size: 160 kDa<br /><br />Exposure time: 23 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-80" style="font-weight: bold;text-decoration: underline;">ET1610-80</a>) at 1/5,000 dilution was used in antibody dilution buffer (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of APC on different lysates with Rabbit anti-APC antibody (ET1610-80) at 1/5,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: C2C12 cell lysate
    Lane 3: C6 cell lysate
    Lane 4: mouse brain tissue lysate
    Lane 5: rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 160 kDa
    Observed band size: 160 kDa

    Exposure time: 23 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-80) at 1/5,000 dilution was used in antibody dilution buffer (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation