ARL13B Mouse Monoclonal Antibody [A4E4]
Catalog# HA600003
ARL13B Mouse Monoclonal Antibody [A4E4]
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WB
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Human
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unconjugated
Overview
Product Name
ARL13B Mouse Monoclonal Antibody [A4E4]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within human ARL13B aa 370-428.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 49 kDa
Positive Control
293T cell lysate, HL-60 cell lysate.
Conjugation
unconjugated
Clone Number
A4E4
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
Target
Function
Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze it; the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development: required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling. Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex. May regulate endocytic recycling traffic; however, additional evidence is required to confirm these data.
Background References
1. Hori Y. et. al. Domain architecture of the atypical Arf-family GTPase Arl13b involved in cilia formation. Biochem. Biophys. Res. Commun. 373:119-124 (2008).
Subcellular Location
Cell membrane, Cell projection, Cilium, Membrane.
UNIPROT
Synonyms
ADP ribosylation factor like 13B antibody
ADP ribosylation factor like 2 like 1 antibody
ADP-ribosylation factor-like protein 13B antibody
ADP-ribosylation factor-like protein 2-like 1 antibody
AR13B_HUMAN antibody
arl13b antibody
ARL2-like protein 1 antibody
ARL2L1 antibody
JBTS8 antibody
Images
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Western blot analysis of ARL13B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600003, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293T cell lysate
Lane 2: HL-60 cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"