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ASC1 Recombinant Rabbit Monoclonal Antibody [PSH0-26] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750600

ASC1 Recombinant Rabbit Monoclonal Antibody [PSH0-26] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ASC1 Recombinant Rabbit Monoclonal Antibody [PSH0-26] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ASC1 aa 1-581 / 581.

Species Reactivity

Human, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 66 kDa

Positive Control

A549 cell lysate, Hela cell lysate, U87-MG cell lysate, 293T cell lysate, HL-60 cell lysate, HepG2 cell lysate, RH-35 cell lysate, HeLa cell lysate, Rat testis tissue lysate, rat small intestine tissue.

Conjugation

unconjugated

Clone Number

PSH0-26

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

This gene encodes a subunit of the tetrameric nuclear activating signal cointegrator 1 (ASC-1) complex, which associates with transcriptional coactivators, nuclear receptors and basal transcription factors to facilitate nuclear receptors-mediated transcription. This protein is localized in the nucleus and contains an E1A-type zinc finger domain, which mediates interaction with transcriptional coactivators and ligand-bound nuclear receptors, such as thyroid hormone receptor and retinoid X receptor alpha, but not glucocorticoid receptor. Mutations in this gene are associated with spinal muscular atrophy with congenital bone fractures-1 (SMABF1).

Background References

1. Dembour A et al. ASC1 complex related conditions: Two novel paediatric patients with TRIP4 pathogenic variants and review of literature. Eur J Med Genet. 2022 Apr

Subcellular Location

Nucleus, Cytoplasm, cytosol, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

UNIPROT

SwissProt: Q15650 Human

Entrez Gene: 315769 Rat

Synonyms

Activating signal cointegrator 1 antibody

ASC 1 antibody

ASC-1 antibody

HsT17391 antibody

Thyroid hormone receptor interactor 4 antibody

Thyroid receptor interacting protein 4 antibody

Thyroid receptor-interacting protein 4 antibody

TR-interacting protein 4 antibody

TRIP 4 antibody

TRIP-4 antibody

Expand

Images

  • Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (<a href="/products/HA750600" style="font-weight: bold;text-decoration: underline;">HA750600</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate<br />Lane 2: Hela cell lysate<br />Lane 3: U87-MG cell lysate<br />Lane 4: 293T cell lysate<br />Lane 5: HL-60 cell lysate<br />Lane 6: HepG2 cell lysate<br />Lane 7: RH-35 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 66 kDa<br />Observed band size: 66 kDa<br /><br />Exposure time: 3 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750600" style="font-weight: bold;text-decoration: underline;">HA750600</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (HA750600) at 1/1,000 dilution.

    Lane 1: A549 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: U87-MG cell lysate
    Lane 4: 293T cell lysate
    Lane 5: HL-60 cell lysate
    Lane 6: HepG2 cell lysate
    Lane 7: RH-35 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

    Exposure time: 3 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750600) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (<a href="/products/HA750600" style="font-weight: bold;text-decoration: underline;">HA750600</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293T cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: HepG2 cell lysate (20 µg/Lane)<br />Lane 4: Rat testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 66 kDa<br />Observed band size: 66 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750600" style="font-weight: bold;text-decoration: underline;">HA750600</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (HA750600) at 1/1,000 dilution.

    Lane 1: 293T cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: HepG2 cell lysate (20 µg/Lane)
    Lane 4: Rat testis tissue lysate (40 µg/Lane)

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750600) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-ASC1 antibody (<a href="/products/HA750600" style="font-weight: bold;text-decoration: underline;">HA750600</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750600" style="font-weight: bold;text-decoration: underline;">HA750600</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-ASC1 antibody (HA750600) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750600) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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ASC1 Recombinant Rabbit Monoclonal Antibody [PSH0-26]

Application: WB,IHC-P

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Conjugate: unconjugated