ATG16L1 Mouse Monoclonal Antibody [A11-H9]
Catalog# EM1708-14
ATG16L1 Mouse Monoclonal Antibody [A11-H9]
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WB
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ATG16L1 Mouse Monoclonal Antibody [A11-H9]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human ATG16L1 aa 11-257/607.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Molecular Weight
68 kDa
Positive Control
Human ATG16L1 recombinant protein, ATG16L1-hIgGFc transfected HEK293 cell lysate, Hela, Raji, PANC-1, Jurkat, PC-12 , HepG2, Hek293, NIH3T3.
Conjugation
unconjugated
Clone Number
A11-H9
Product Features
Form
Liquid
Concentration
Storage Instructions
4℃; -20℃ for long term storage.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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FC
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1:100-1:200
Target
Function
Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
Background References
1. Raju D et al. Crohn disease ATG16L1 polymorphism increases susceptibility to infection with Helicobacter pylori in humans. Autophagy 8(9):1387-8 (2012).
2. Fabio RR et al.ATG16L1 contribution to Crohn\'s disease risk in Sicily.Inflamm Bowel Dis 17(7):1635-6 (2011).
Subcellular Location
Cytoplasm.
Synonyms
A16L1_HUMAN antibody
APG16 like 1 antibody
APG16-like 1 antibody
APG16L antibody
APG16L beta antibody
ATG16 autophagy related 16 like 1 antibody
ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody
ATG16A antibody
ATG16L antibody
Atg16l1 antibody
ExpandImages
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Western blot analysis of ATG16L1 on human ATG16L1 recombinant protein using anti-ATG16L1 antibody at 1/1,000 dilution.
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Western blot analysis of ATG16L1 on HEK293 (1) and ATG16L1-hIgGFc transfected HEK293 (2) cell lysate using anti-ATG16L1 antibody at 1/1,000 dilution.
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Western blot analysis of ATG16L1 on different cell lysate using anti-ATG16L1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Raji
Lane 3: PANC-1
Lane 4: Jurkat
Lane 5: PC-12
Lane 6: HepG2
Lane 7: Hek293
Lane 8: NIH3T3
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
ATG16L1 Recombinant Rabbit Monoclonal Antibody [JU30-62]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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