ATG4B Rabbit Polyclonal Antibody
Catalog# ER62759
ATG4B Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Human
-
Mouse
-
Rat
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Green monkey
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unconjugated
Overview
Product Name
ATG4B Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human ATG4B aa 1-393 / 393 (Q9Y4P1).
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 44 kDa
Positive Control
293T cell lysate, MCF7 cell lysate, COS-1 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human colon cancer tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IHC-P
-
1:1,000
Target
Function
Cysteine protease ATG4B is an enzyme that in humans is encoded by the ATG4B gene. Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. One main function of Atg4 is to cleave the pre-protein of Atg8, leading to the non-lipidated soluble (-I) form which can be processed further by Atg3, Atg7, Atg5-12 into the lipidated form (-II) anchored to the autophagic membrane.
Background References
1. Sun L et al. Deacetylation of ATG4B promotes autophagy initiation under starvation. Sci Adv. 2022 Aug
2. Yang G et al. Targeting Atg4B for cancer therapy: Chemical mediators. Eur J Med Chem. 2021 Jan
Subcellular Location
Cytoplasm, cytosol, Cytoplasmic vesicle, autophagosome, Endoplasmic reticulum, Mitochondrion.
Synonyms
APG4 autophagy 4 homolog B antibody
APG4B antibody
ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
ATG4B antibody
ATG4B_HUMAN antibody
AUT like 1 cysteine endopeptidase antibody
AUT-like 1 cysteine endopeptidase antibody
AUTL1 antibody
Autophagin 1 antibody
Autophagin-1 antibody
ExpandImages
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Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (ER62759) at 1/2,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: COS-1 cell lysate (20 µg/Lane)
Lane 4: PC-12 cell lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 44 kDa
Observed band size: 47 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER62759) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ATG4B antibody (ER62759) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER62759) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Application: WB
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
