AU1 tag Mouse Monoclonal Antibody [F7-C2]
Catalog# EM50803
AU1 tag Mouse Monoclonal Antibody [F7-C2]
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WB
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ELISA
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IP
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IF-Cell
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Species independent
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unconjugated
Overview
Product Name
AU1 tag Mouse Monoclonal Antibody [F7-C2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
This antibody is produced by immunizing rabbits with a synthetic peptide DTYRYI (KLH-coupled).
Species Reactivity
Species independent
Validated Applications
WB, ELISA, IP, IF-Cell
Positive Control
AU1-tagged recombinant protein.
Conjugation
unconjugated
Clone Number
F7-C2
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein L affinity purified.
Application Dilution
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WB
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1:2,000
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ELISA
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Use at an assay dependent concentration.
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IF-Cell
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Use at an assay dependent concentration.
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IP
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Use at an assay dependent concentration.
Target
Function
The recognized AU1 sequence represents the amino acid sequence DTYRYI, derived from the major capsid protein of bovine papillomavirus-1 (BPV-1). AU1 is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized by immunochemical methods. This antibody recognizes the AU1-tag fused to the carboxyl terminus of targeted proteins.
Background References
1. Jones DT., et al. Recurrent somatic alterations of FGFR1 and NTRK2 in pilocytic astrocytoma. Nat Genet45:927-32 (2013)
2. Avbelj M.,et al. The role of intermediary domain of MyD88 in cell activation and therapeutic inhibition of TLRs. J Immunol. 187:2394-404 (2011).
Synonyms
AU1 epitope tag antibody
DTYRYI epitope tag antibody
DTYRYI tag antibody
Images
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Western blot analysis of AU1 tag on AU1-tagged recombinant protein using anti-AU1 tag antibody at 1/2,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"