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Adipose Triglyceride Lipase Recombinant Rabbit Monoclonal Antibody [JE37-49]

Usd: 385

Specification

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Catalog# HA721951

Adipose Triglyceride Lipase Recombinant Rabbit Monoclonal Antibody [JE37-49]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Adipose Triglyceride Lipase Recombinant Rabbit Monoclonal Antibody [JE37-49]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Adipose Triglyceride Lipase aa 330-450 / 504.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 55 kDa

Positive Control

SiHa cell lysate, HepG2 cell lysate, HEK-293 cell lysate, A431 cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate, Mouse liver tissue lysate, Mouse white adipose tissue lysate, Mouse brown adipose tissue lysate, Rat brown adipose tissue lysate, mouse brown adipose tissue, rat breast tissue.

Conjugation

unconjugated

Clone Number

JE37-49

RRID

AB_3096523

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:500-1:2,000

  • IF-Tissue

  • 1:200

Target

Function

Adipose triglyceride lipase, also known as patatin-like phospholipase domain-containing protein 2 and ATGL, is an enzyme that in humans is encoded by the PNPLA2 gene. ATGL catalyses the first reaction of lipolysis, where triacylglycerols are hydrolysed to diacylglycerols. ATGL has very high substrate specificity for triacylglycerols. It contains a catalytic dyad using serine-aspartic acid. ATGL catalyses the first reaction of lipolysis. It hydrolysis triacylglycerols to diacylglycerols by attacking the fatty acid attached to carbon-3 of glycerol. ATGL acts as a control mechanism of lipolysis, as variations in diacylglycerol concentration impact enzymes in later stages of lipolysis.

Background References

1. Fuchs CD et al. Hepatocyte-specific deletion of adipose triglyceride lipase (adipose triglyceride lipase/patatin-like phospholipase domain containing 2) ameliorates dietary induced steatohepatitis in mice. Hepatology. 2022 Jan

2. Li T et al. Adipose Triglyceride Lipase in Hepatic Physiology and Pathophysiology. Biomolecules. 2021 Dec

Subcellular Location

Lipid droplet, Cell membrane, Cytoplasm.

UNIPROT

SwissProt: Q96AD5 Human

SwissProt: Q8BJ56 Mouse

SwissProt: P0C548 Rat

Synonyms

1110001C14Rik antibody

Adipose triglyceride lipase antibody

ATGL antibody

ATGL DESNUTRIN antibody

Calcium independent phospholipase A2 antibody

Calcium-independent phospholipase A2 antibody

Desnutrin antibody

EC 3.1.1.3 antibody

FP17548 antibody

IPLA2 zeta antibody

Expand

Images

  • Western blot analysis of Adipose Triglyceride Lipase on different lysates with Rabbit anti-Adipose Triglyceride Lipase antibody (<a href="/products/HA721951" style="font-weight: bold;text-decoration: underline;">HA721951</a>) at 1/1,000 dilution.<br /><br />Lane 1: SiHa cell lysate (30 µg/Lane)<br />Lane 2: HepG2 cell lysate (30 µg/Lane)<br />Lane 3: HEK-293 cell lysate (30 µg/Lane)<br />Lane 4: A431 cell lysate (30 µg/Lane)<br />Lane 5: Mouse heart tissue lysate (40 µg/Lane)<br />Lane 6: Rat heart tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 55 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 5 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721951" style="font-weight: bold;text-decoration: underline;">HA721951</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Adipose Triglyceride Lipase on different lysates with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/1,000 dilution.

    Lane 1: SiHa cell lysate (30 µg/Lane)
    Lane 2: HepG2 cell lysate (30 µg/Lane)
    Lane 3: HEK-293 cell lysate (30 µg/Lane)
    Lane 4: A431 cell lysate (30 µg/Lane)
    Lane 5: Mouse heart tissue lysate (40 µg/Lane)
    Lane 6: Rat heart tissue lysate (40 µg/Lane)

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

    Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 5 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721951) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Adipose Triglyceride Lipase on different lysates with Rabbit anti-Adipose Triglyceride Lipase antibody (<a href="/products/HA721951" style="font-weight: bold;text-decoration: underline;">HA721951</a>) at 1/1,000 dilution.<br /><br />Lane 1: A431 cell lysate (30 µg/Lane)<br />Lane 2: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 3: Mouse white adipose tissue lysate (40 µg/Lane)<br />Lane 4: Mouse brown adipose tissue lysate (40 µg/Lane)<br />Lane 5: Rat heart tissue lysate (40 µg/Lane)<br />Lane 6: Rat brown adipose tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 55 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721951" style="font-weight: bold;text-decoration: underline;">HA721951</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Adipose Triglyceride Lipase on different lysates with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/1,000 dilution.

    Lane 1: A431 cell lysate (30 µg/Lane)
    Lane 2: Mouse liver tissue lysate (40 µg/Lane)
    Lane 3: Mouse white adipose tissue lysate (40 µg/Lane)
    Lane 4: Mouse brown adipose tissue lysate (40 µg/Lane)
    Lane 5: Rat heart tissue lysate (40 µg/Lane)
    Lane 6: Rat brown adipose tissue lysate (40 µg/Lane)

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721951) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-Adipose Triglyceride Lipase antibody (<a href="/products/HA721951" style="font-weight: bold;text-decoration: underline;">HA721951</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721951" style="font-weight: bold;text-decoration: underline;">HA721951</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721951) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat breast tissue with Rabbit anti-Adipose Triglyceride Lipase antibody (<a href="/products/HA721951" style="font-weight: bold;text-decoration: underline;">HA721951</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721951" style="font-weight: bold;text-decoration: underline;">HA721951</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat breast tissue with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721951) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling Adipose Triglyceride Lipase with Rabbit anti-Adipose Triglyceride Lipase antibody (<a href="/products/HA721951" style="font-weight: bold;text-decoration: underline;">HA721951</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA721951" style="font-weight: bold;text-decoration: underline;">HA721951</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling Adipose Triglyceride Lipase with Rabbit anti-Adipose Triglyceride Lipase antibody (HA721951) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721951, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

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Citation