Aldehyde Oxidase Recombinant Rabbit Monoclonal Antibody [PSH0-17] - BSA and Azide free
Catalog# HA750589
Aldehyde Oxidase Recombinant Rabbit Monoclonal Antibody [PSH0-17] - BSA and Azide free
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Aldehyde Oxidase Recombinant Rabbit Monoclonal Antibody [PSH0-17] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human AOX1 aa 1,301-1,338 / 1,338.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 148 kDa
Positive Control
Rat liver tissue lysates, rat liver tissue, rat adrenal gland tissue, human kidney tissue, mouse testis tissue, Hela.
Conjugation
unconjugated
Clone Number
PSH0-17
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Aldehyde oxidase 1 is an enzyme that in humans is encoded by the AOX1 gene. Aldehyde oxidase produces hydrogen peroxide and, under certain conditions, can catalyze the formation of superoxide. Aldehyde oxidase is a candidate gene for amyotrophic lateral sclerosis. Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as N1-methylnicotinamide, N-methylphthalazinium and phthalazine, as well as aldehydes, such as benzaldehyde, retinal, pyridoxal, and vanillin. Plays a key role in the metabolism of xenobiotics and drugs containing aromatic azaheterocyclic substituents. Participates in the bioactivation of prodrugs such as famciclovir, catalyzing the oxidation step from 6-deoxypenciclovir to penciclovir, which is a potent antiviral agent. Is probably involved in the regulation of reactive oxygen species homeostasis. May be a prominent source of superoxide generation via the one-electron reduction of molecular oxygen. Also may catalyze nitric oxide (NO) production via the reduction of nitrite to NO with NADH or aldehyde as electron donor. May play a role in adipogenesis.
Background References
1. Xiong L et al. Expression of AOX1 Predicts Prognosis of Clear Cell Renal Cell Carcinoma. Front Genet. 2021 Jul
2. Yu Y et al. Clinical implications of TPO and AOX1 in pediatric papillary thyroid carcinoma. Transl Pediatr. 2021 Apr
Subcellular Location
Cytoplasm.
Synonyms
Aldehyde oxidase 1 antibody
AO antibody
AOH1 antibody
AOX 1 antibody
azaheterocycle hydroxylase antibody
EC 1.2.3.1 antibody
Images
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This data was developed using HA721277, the same antibody clone in a different buffer formulation.
Western blot analysis of Aldehyde Oxidase on rat liver lysate with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/1,000 dilution.
Lysates/proteins at 40 µg/Lane.
Exposure time: 41 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721277, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 148 kDa
Observed band size: 148 kDa -
This data was developed using HA721277, the same antibody clone in a different buffer formulation.
Western blot analysis of Aldehyde Oxidase on mouse liver lysate with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/1,000 dilution.
Lysates/proteins at 40 µg/Lane.
Exposure time: 41 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721277, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 148 kDa
Observed band size: 148 kDa -
This data was developed using HA721277, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721277) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721277, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721277) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721277, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721277) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721277, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721277) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721277, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Hela cells labeling Aldehyde Oxidase.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721277, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Aldehyde Oxidase Recombinant Rabbit Monoclonal Antibody [PSH0-17]
Application: WB,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
