Aldolase C Recombinant Rabbit Monoclonal Antibody [PSH01-82] - BSA and Azide free
Catalog# HA750756
Aldolase C Recombinant Rabbit Monoclonal Antibody [PSH01-82] - BSA and Azide free
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WB
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IHC-P
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Mouse
-
Rat
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Human
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unconjugated
Overview
Product Name
Aldolase C Recombinant Rabbit Monoclonal Antibody [PSH01-82] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Aldolase C aa 50-100.
Species Reactivity
Mouse, Rat (Predicted: Human)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
Mouse brain tissue lysate, mouse hippocampus tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, mouse brain tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
PSH01-82
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IHC-P
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1:5,000
Target
Function
ALDOC is a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, Aldolase C is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively.
Background References
1. Chen L, Zeng Y, Ren B, et al. ALDOC regulated the biological function and immune infiltration of gastric cancer cells. Int J Biochem Cell Biol. 2023 May; 158:106407.
2. Fan K, Wang J, Sun W, et al. MUC16 C-terminal binding with ALDOC disrupts the ability of ALDOC to sense glucose and promotes gallbladder carcinoma growth. Exp Cell Res. 2020 Sep 1;394(1):112118.
Subcellular Location
cytoskeleton, cytosol, extracellular exosome, extracellular region
Synonyms
ALDC antibody
ALDO C antibody
aldoc antibody
ALDOC_HUMAN antibody
Aldolase 3 antibody
Aldolase C Fructose bisphosphate antibody
Brain type aldolase antibody
Brain-type aldolase antibody
Fructoaldolase C antibody
Fructose 1 6 biphosphate triosephosphate lyase antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of Aldolase C on different lysates with Rabbit anti-Aldolase C antibody (HA750756) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse hippocampus tissue lysate
Lane 3: Mouse heart tissue lysate (low expression)
Lane 4: Mouse pancreas tissue lysate (negative)
Lane 5: Rat brain tissue lysate
Lane 6: Rat hippocampus tissue lysate
Lane 7: Rat heart tissue lysate (low expression)
Lane 8: Rat pancreas tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750756) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Aldolase C antibody (HA750756) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750756) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Aldolase C antibody (HA750756) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750756) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue (negative) with Rabbit anti-Aldolase C antibody (HA750756) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750756) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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