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Alkaline Phosphatase Mouse Monoclonal Antibody [6-10]

Usd: 350

Specification

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Catalog# M0806-10

Alkaline Phosphatase Mouse Monoclonal Antibody [6-10]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Alkaline Phosphatase Mouse Monoclonal Antibody [6-10]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within human Alkaline Phosphatase aa 350-450.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, FC

Molecular Weight

58 kDa

Positive Control

Hela, mouse ovary

Conjugation

unconjugated

Clone Number

6-10

RRID

AB_3072988

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000-1:10,000

  • IHC-P

  • 1:200

Target

Function

Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Placental ALP is highly polymorphic, there are at least three common alleles. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Background References

1. Ezra E., Blacher R., Udenfriend S.; "Purification and partial sequencing of human placental alkaline phosphatase."; Biochem. Biophys. Res. Commun. 116:1076-1083(1983).

2. Garattini E., Hua J.-C., Pan Y.C.E., Udenfriend S.; "Human liver alkaline phosphatase, purification and partial sequencing: homology with the placental isozyme."; Arch. Biochem. Biophys. 245:331-337(1986).

Sequence Similarity

Belongs to the alkaline phosphatase family.

Tissue Specificity

Detected in placenta (at protein level).

Subcellular Location

cell membrane

UNIPROT

SwissProt: P05187 Human

SwissProt: P24823 Mouse

Synonyms

Alkaline phosphatase antibody

Alkaline phosphatase placental antibody

Alkaline phosphatase placental type antibody

Alkaline phosphatase Regan isozyme antibody

ALP antibody

Alp1 antibody

ALPP antibody

FLJ61142 antibody

Germ-cell alkaline phosphatase antibody

nagao isozyme antibody

Expand

Images

  • Western blot analysis on Hela cell lysates using anti-Alkaline Phosphatase Mouse mAb (Cat. # <a href="/products/M0806-10" style="font-weight: bold;text-decoration: underline;">M0806-10</a>).

    Western blot analysis on Hela cell lysates using anti-Alkaline Phosphatase Mouse mAb (Cat. # M0806-10).

  • Immunohistochemical analysis of paraffin- embedded mouse ovary tissue anti-Alkaline Phosphatase Mouse mAb (Cat. # <a href="/products/M0806-10" style="font-weight: bold;text-decoration: underline;">M0806-10</a>).

    Immunohistochemical analysis of paraffin- embedded mouse ovary tissue anti-Alkaline Phosphatase Mouse mAb (Cat. # M0806-10).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation