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Alpha 1 Acid Glycoprotein Recombinant Mouse Monoclonal Antibody [A4-G2-A3-R]

Usd: 350

Specification

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Catalog# HA601226

Alpha 1 Acid Glycoprotein Recombinant Mouse Monoclonal Antibody [A4-G2-A3-R]

Application

  • WB

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Alpha 1 Acid Glycoprotein Recombinant Mouse Monoclonal Antibody [A4-G2-A3-R]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human ORM1 aa 1-201 / 201.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 24 kDa

Positive Control

HepG2 cell lysate, HeLa cell lysate, HCT 116 cell lysate, 293T cell lysate, human liver tissue lysate.

Conjugation

unconjugated

Clone Number

A4-G2-A3-R

RRID

AB_3096658

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression. Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.

Background References

1. Fitos I et al. Selective binding of imatinib to the genetic variants of human alpha1-acid glycoprotein. Biochim Biophys Acta 1760:1704-1712(2006).

2. Zsila F et al. The drug binding site of human alpha1-acid glycoprotein: insight from induced circular dichroism and electronic absorption spectra. Biochim Biophys Acta 1770:797-809(2007).

Subcellular Location

Secreted.

UNIPROT

SwissProt: P02763 Human

Synonyms

A1AG1_HUMAN antibody

AGP 1 antibody

AGP A antibody

AGP antibody

AGP1 antibody

Alpha 1 acid glycoprotein antibody

Alpha-1-acid glycoprotein 1 antibody

alpha-1-AGP antibody

Epididymis secretory sperm binding protein Li 153w antibody

glycoprotein, alpha-1-acid, of serum antibody

Expand

Images

  • Western blot analysis of Alpha 1 Acid Glycoprotein on different lysates with Mouse anti-Alpha 1 Acid Glycoprotein antibody (<a href="/products/HA601226" style="font-weight: bold;text-decoration: underline;">HA601226</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: HCT 116 cell lysate (20 µg/Lane)<br />Lane 4: 293T cell lysate (20 µg/Lane)<br />Lane 5: Human liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 24 kDa<br />Observed band size: 37/45 kDa<br /><br />Exposure time: 43 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601226" style="font-weight: bold;text-decoration: underline;">HA601226</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Alpha 1 Acid Glycoprotein on different lysates with Mouse anti-Alpha 1 Acid Glycoprotein antibody (HA601226) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: HCT 116 cell lysate (20 µg/Lane)
    Lane 4: 293T cell lysate (20 µg/Lane)
    Lane 5: Human liver tissue lysate (40 µg/Lane)

    Predicted band size: 24 kDa
    Observed band size: 37/45 kDa

    Exposure time: 43 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601226) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"