Alpha-2-macroglobulin Mouse Monoclonal Antibody [B1-D7]
Catalog# M1510-24
Alpha-2-macroglobulin Mouse Monoclonal Antibody [B1-D7]
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WB
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IHC-P
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ELISA
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Human
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unconjugated
Overview
Product Name
Alpha-2-macroglobulin Mouse Monoclonal Antibody [B1-D7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human Alpha-2-macroglobulin aa 950-1200.
Species Reactivity
Human
Validated Applications
WB, IHC-P, ELISA
Molecular Weight
Predicted band size: 163 kDa
Positive Control
LO2 cell lysates, human endometrium tissue, human testis tissue, human liver tissue.
Conjugation
unconjugated
Clone Number
B1-D7
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:2,000
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ELISA
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Use at an assay dependent concentration.
Target
Function
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Background References
1. Sottrup-Jensen L et al. Primary structure of human alpha 2-macroglobulin. V. The complete structure. J Biol Chem 259:8318-8327 (1984).
2. Liu T et al. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. J Proteome Res 4:2070-2080 (2005).
Sequence Similarity
Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
Tissue Specificity
Secreted in plasma.
Subcellular Location
Secreted.
UNIPROT
Synonyms
A2m antibody
A2MG_HUMAN antibody
Alpha 2 M antibody
Alpha 2M antibody
Alpha-2-M antibody
Alpha-2-macroglobulin antibody
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
CPAMD5 antibody
DKFZp779B086 antibody
FWP007 antibody
ExpandImages
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Western blot analysis of Alpha-2-macroglobulin on LO2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1510-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Mouse anti-Alpha-2-macroglobulin antibody (M1510-24) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-24) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-Alpha-2-macroglobulin antibody (M1510-24) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-24) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Alpha-2-macroglobulin antibody (M1510-24) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-24) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Alpha-2-macroglobulin Mouse Monoclonal Antibody [A5-A9]
Application: WB,IF-Cell,IHC-P,ELISA
Reactivity: Human
Conjugate: unconjugated
