Annexin A1 Rabbit Polyclonal Antibody
Catalog# ER1803-70
Annexin A1 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Annexin A1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human Annexin A1 aa 150-310.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC, IP
Target Molecular Weight
Predicted band size: 39 kDa
Positive Control
A431 cell lysate, K-562 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, A431, NIH/3T3, C6, human esophagus tissue, human placenta tissue, mouse esophagus tissue, rat esophagus tissue.
Conjugation
unconjugated
RRID
Reactivity Data
| WB | IF-Cell | IHC-P | FC | IP | |
|---|---|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IF-Cell
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1:100-1:250
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IHC-P
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1:200-1:1,000
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FC
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1:1,000
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IP
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1-2μg/sample
Target
Function
Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Promotes resolution of inflammation and wound healing. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T cells. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca2+ and can bind up to eight Ca2+ ions. Displays Ca2+-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca2+-dependent interaction between phagosomes and the actin cytoskeleton.
Background References
1. Wallner B P et al. Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity. Nature 320:77-81 (1986).
2. Arcone R et al. Structural characterization of a biologically active human lipocortin 1 expressed in Escherichia coli. Eur J Biochem 211:347-355 (1993).
Sequence Similarity
Belongs to the annexin family.
Tissue Specificity
Detected in resting neutrophils. Detected in peripheral blood T-cells. Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors. Detected in placenta (at protein level). Detected in liver.
Post-translational Modification
Phosphorylated by protein kinase C, EGFR and TRPM7. Phosphorylated in response to EGF treatment.; Sumoylated.
Subcellular Location
Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane, Nucleus, Secreted.
Synonyms
Annexin 1 antibody
Annexin A1 antibody
Annexin I (lipocortin I) antibody
Annexin I antibody
Annexin-1 antibody
AnnexinA1 antibody
AnnexinI antibody
ANX 1 antibody
ANX A1 antibody
ANX1 antibody
ExpandImages
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Western blot analysis of Annexin A1 on different lysates with Rabbit anti-Annexin A1 antibody (ER1803-70) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: K-562 cell lysate
Lane 3: C2C12 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 33/37 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-70) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A431 cells labeling Annexin A1 with Rabbit anti-Annexin A1 antibody (ER1803-70) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Annexin A1 antibody (ER1803-70) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of NIH/3T3 cells labeling Annexin A1 with Rabbit anti-Annexin A1 antibody (ER1803-70) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Annexin A1 antibody (ER1803-70) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of C6 cells labeling Annexin A1 with Rabbit anti-Annexin A1 antibody (ER1803-70) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Annexin A1 antibody (ER1803-70) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-Annexin A1 antibody (ER1803-70) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-70) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Annexin A1 antibody (ER1803-70) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-70) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue with Rabbit anti-Annexin A1 antibody (ER1803-70) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-70) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-Annexin A1 antibody (ER1803-70) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-70) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of A431 cells labeling Annexin A1.
Cells were fixed and permeabilized. Then stained with the primary antibody (ER1803-70, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Annexin A1 was immunoprecipitated from 0.2 mg A431 cell lysate with ER1803-70 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ER1803-70 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate (input)
Lane 2: ER1803-70 IP in A431 cell lysate
Lane 3: Rabbit IgG instead of ER1803-70 in A431 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 1 seconds; ECL: K1801
Citation
-
LXR-β regulates microglial efferocytosis and neuroinflammation in CPSP via STAT6 activation
Journal: Brain, Behavior, And Immunity
DOI: 10.1016/j.bbi.2025.106089
IF: 7.6
Application: WB
Reactivity: Mouse
Publish date: 2025 Aug
-
Mannose modified graphene oxide drug-delivery system targets cancer stem cells and tumor-associated macrophages to promote immunotherapeutic efficacy
Journal: Colloids And Surfaces B: Biointerfaces
DOI: 10.1016/j.colsurfb.2025.114710
IF: 5.4
Application: WB
Reactivity: Mouse
Publish date: 2025 Apr
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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