Annexin A3 Recombinant Mouse Monoclonal Antibody [A1F7-R]
Catalog# HA601303
Annexin A3 Recombinant Mouse Monoclonal Antibody [A1F7-R]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Annexin A3 Recombinant Mouse Monoclonal Antibody [A1F7-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human Annexin A3 aa 1-323 / 323.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 36 kDa
Positive Control
A431 cell lysate, HEK-293 cell lysate, HepG2 cell lysate, HeLa cell lysate, HT-29 cell lysate, MCF7 cell lysate, human lung tissue lysate, mouse lung tissue lysate.
Conjugation
unconjugated
Clone Number
A1F7-R
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Annexin A3 is a protein that in humans is encoded by the ANXA3 gene. It is abnormally expressed in fetuses of both IVF and ICSI, which may contribute to the increase risk of birth defects in these ART. This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phospholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.
Background References
1. Wang Y. et. al. ANXA3 Silencing Ameliorates Intracranial Aneurysm via Inhibition of the JNK Signaling Pathway. Mol Ther Nucleic Acids. 2019 Sep 6;17:540-550.
2. Wang L. et. al. Cancer-associated fibroblasts contribute to cisplatin resistance by modulating ANXA3 in lung cancer cells. Cancer Sci. 2019 May;110(5):1609-1620.
Subcellular Location
Cytosol, extracellular exosome, plasma membrane, axon, cytoplasm, dendrite, membrane, neuronal cell body, phagocytic vesicle membrane, specific granule.
Synonyms
2-cyclic phosphate 2-phosphohydrolase antibody
35-alpha calcimedin antibody
Annexin A3 antibody
Annexin III antibody
Annexin-3 antibody
ANX3 antibody
ANXA 3 antibody
Anxa3 antibody
ANXA3_HUMAN antibody
Calcimedin 35 alpha antibody
ExpandImages
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Western blot analysis of Annexin A3 on different lysates with Mouse anti-Annexin A3 antibody (HA601303) at 1/2,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: HT-29 cell lysate (20 µg/Lane)
Lane 6: MCF7 cell lysate (20 µg/Lane)
Lane 7: Human lung tissue lysate (40 µg/Lane)
Lane 8: Mouse lung tissue lysate (40 µg/Lane)
Predicted band size: 36 kDa
Observed band size: 33/36 kDa
Exposure time: 12 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601303) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Annexin A3 Recombinant Mouse Monoclonal Antibody [A1F7-R] - BSA and Azide free
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Annexin A3 Mouse Monoclonal Antibody [A1F7]
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
